CRISPR-U™ Gene Knockout Cell Line
Strategy
EIF3H Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human EIF3H Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | EIF3H |
| Gene id | 8667 |
| Organism | Homo sapiens |
| Official full symbol | eukaryotic translation initiation factor 3 subunit H |
| Gene type | protein-coding |
| Also known as | EIF3S3, eIF3-gamma, eIF3-p40 |
| Genomic regions | Chromosome 8 |
Strategy Summary
This gene has 7 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| EIF3H-209 | ENST00000521861.6 | 3961 | 352aa | Protein coding | CCDS6319 | O15372 Q6IB98 | NM_003756.3 | TSL:1, GENCODE basic, APPRIS P2, MANE Select v0.92, |
| EIF3H-201 | ENST00000276682.8 | 2041 | 366aa | Protein coding | - | B3KS98 | - | TSL:2, GENCODE basic, |
| EIF3H-212 | ENST00000611080.1 | 1265 | 349aa | Protein coding | - | A0A087WZK9 | - | TSL:5, GENCODE basic, APPRIS ALT1, |
| EIF3H-204 | ENST00000518949.5 | 843 | 244aa | Protein coding | - | E5RJT0 | - | CDS 3' incomplete, TSL:3, |
| EIF3H-205 | ENST00000518995.5 | 731 | 235aa | Protein coding | - | E5RGU4 | - | CDS 3' incomplete, TSL:3, |
| EIF3H-208 | ENST00000520813.1 | 562 | 97aa | Protein coding | - | E5RFW7 | - | CDS 3' incomplete, TSL:4, |
| EIF3H-210 | ENST00000522453.1 | 518 | 170aa | Protein coding | - | E5RFH0 | - | CDS 3' incomplete, TSL:3, |
| EIF3H-211 | ENST00000522800.5 | 635 | 56aa | Nonsense mediated decay | - | E5RH59 | - | TSL:3, |
| EIF3H-206 | ENST00000519046.5 | 488 | 46aa | Nonsense mediated decay | - | E5RHC7 | - | TSL:3, |
| EIF3H-203 | ENST00000518034.5 | 1047 | No protein | Retained intron | - | - | - | TSL:2, |
| EIF3H-207 | ENST00000520289.1 | 855 | No protein | Retained intron | - | - | - | TSL:2, |
| EIF3H-202 | ENST00000517974.1 | 594 | No protein | Retained intron | - | - | - | TSL:2, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | EIF3H |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene EIF3H had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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