CRISPR-U™ Gene Knockout Cell Line Strategy

EIF3H Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human EIF3H Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol EIF3H
Gene id 8667
Organism Homo sapiens
Official full symbol eukaryotic translation initiation factor 3 subunit H
Gene type protein-coding
Also known as EIF3S3, eIF3-gamma, eIF3-p40
Genomic regions Chromosome 8
Strategy Summary
This gene has 7 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
EIF3H-209 ENST00000521861.6 3961 352aa Protein coding CCDS6319 O15372 Q6IB98 NM_003756.3 TSL:1, GENCODE basic, APPRIS P2, MANE Select v0.92,
EIF3H-201 ENST00000276682.8 2041 366aa Protein coding - B3KS98 - TSL:2, GENCODE basic,
EIF3H-212 ENST00000611080.1 1265 349aa Protein coding - A0A087WZK9 - TSL:5, GENCODE basic, APPRIS ALT1,
EIF3H-204 ENST00000518949.5 843 244aa Protein coding - E5RJT0 - CDS 3' incomplete, TSL:3,
EIF3H-205 ENST00000518995.5 731 235aa Protein coding - E5RGU4 - CDS 3' incomplete, TSL:3,
EIF3H-208 ENST00000520813.1 562 97aa Protein coding - E5RFW7 - CDS 3' incomplete, TSL:4,
EIF3H-210 ENST00000522453.1 518 170aa Protein coding - E5RFH0 - CDS 3' incomplete, TSL:3,
EIF3H-211 ENST00000522800.5 635 56aa Nonsense mediated decay - E5RH59 - TSL:3,
EIF3H-206 ENST00000519046.5 488 46aa Nonsense mediated decay - E5RHC7 - TSL:3,
EIF3H-203 ENST00000518034.5 1047 No protein Retained intron - - - TSL:2,
EIF3H-207 ENST00000520289.1 855 No protein Retained intron - - - TSL:2,
EIF3H-202 ENST00000517974.1 594 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene EIF3H
This KO Strategy loading
Red Cotton™ Notes Gene EIF3H had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
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Work flow
Ubigene Red Cotton Workflow

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