CRISPR-U™ Gene Knockout Cell Line
Strategy
LAMTOR3 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human LAMTOR3 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | LAMTOR3 |
| Gene id | 8649 |
| Organism | Homo sapiens |
| Official full symbol | late endosomal/lysosomal adaptor, MAPK and MTOR activator 3 |
| Gene type | protein-coding |
| Also known as | MAP2K1IP1, MAPBP, MAPKSP1, MP1, PRO0633, Ragulator3 |
| Summary | This gene encodes a scaffold protein that functions in the extracellular signal-regulated kinase (ERK) cascade. The protein is localized to late endosomes by the mitogen-activated protein-binding protein-interacting protein, and binds specifically to MAP kinase kinase MAP2K1/MEK1, MAP kinase MAPK3/ERK1, and MAP kinase MAPK1/ERK2. Studies of the orthologous gene in mouse indicate that it regulates late endosomal traffic and cell proliferation. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. A pseudogene of this gene is located on the long arm of chromosome 13. |
| Genomic regions | Chromosome 4 |
Strategy Summary
This gene has 2 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| LAMTOR3-202 | ENST00000499666.7 | 4163 | 124aa | Protein coding | CCDS3652 | Q9UHA4-1 | NM_021970.4 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| LAMTOR3-201 | ENST00000226522.8 | 1220 | 117aa | Protein coding | CCDS58920 | Q9UHA4-2 | - | TSL:3, GENCODE basic, |
| LAMTOR3-203 | ENST00000515100.1 | 961 | No protein | Processed transcript | - | - | - | TSL:1, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | LAMTOR3 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene LAMTOR3 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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