CRISPR-U™ Gene Knockout Cell Line
Strategy
PRKRA Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human PRKRA Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | PRKRA |
| Gene id | 8575 |
| Organism | Homo sapiens |
| Official full symbol | protein activator of interferon induced protein kinase EIF2AK2 |
| Gene type | protein-coding |
| Also known as | DYT16, HSD14, PACT, RAX |
| Summary | This gene encodes a protein kinase activated by double-stranded RNA which mediates the effects of interferon in response to viral infection. Mutations in this gene have been associated with dystonia. Alternative splicing results in multiple transcript variants. |
| Genomic regions | Chromosome 2 |
Strategy Summary
This gene has 7 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| PRKRA-201 | ENST00000325748.9 | 1770 | 313aa | Protein coding | CCDS2279 | O75569-1 | NM_003690.5 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| PRKRA-203 | ENST00000432031.6 | 1344 | 302aa | Protein coding | CCDS46460 | O75569-2 | - | TSL:1, GENCODE basic, |
| PRKRA-211 | ENST00000487082.5 | 1211 | 288aa | Protein coding | CCDS46461 | O75569-3 | - | TSL:1, GENCODE basic, |
| PRKRA-232 | ENST00000678775.1 | 1738 | 200aa | Protein coding | - | B4DJC7 | - | GENCODE basic, |
| PRKRA-224 | ENST00000677689.1 | 1610 | 228aa | Protein coding | - | - | - | GENCODE basic, |
| PRKRA-234 | ENST00000678845.1 | 1546 | 200aa | Protein coding | - | B4DJC7 | - | GENCODE basic, |
| PRKRA-227 | ENST00000677981.1 | 1455 | 229aa | Protein coding | - | - | - | GENCODE basic, |
| PRKRA-205 | ENST00000457633.2 | 2103 | 127aa | Nonsense mediated decay | - | C9JMM3 | - | TSL:3, |
| PRKRA-219 | ENST00000677206.1 | 1999 | 94aa | Nonsense mediated decay | - | - | - | - |
| PRKRA-223 | ENST00000677584.1 | 1885 | 106aa | Nonsense mediated decay | - | - | - | - |
| PRKRA-221 | ENST00000677386.1 | 1824 | 177aa | Nonsense mediated decay | - | - | - | - |
| PRKRA-213 | ENST00000676505.1 | 1793 | 43aa | Nonsense mediated decay | - | - | - | - |
| PRKRA-217 | ENST00000676922.1 | 1780 | 106aa | Nonsense mediated decay | - | - | - | - |
| PRKRA-222 | ENST00000677460.1 | 1752 | 191aa | Nonsense mediated decay | - | - | - | - |
| PRKRA-220 | ENST00000677253.1 | 1717 | 105aa | Nonsense mediated decay | - | - | - | - |
| PRKRA-216 | ENST00000676832.1 | 1713 | 54aa | Nonsense mediated decay | - | - | - | - |
| PRKRA-235 | ENST00000679037.1 | 1709 | 106aa | Nonsense mediated decay | - | - | - | - |
| PRKRA-204 | ENST00000448279.2 | 1661 | 95aa | Nonsense mediated decay | - | F8WEG8 | - | TSL:4, |
| PRKRA-228 | ENST00000678053.1 | 1645 | 54aa | Nonsense mediated decay | - | - | - | - |
| PRKRA-202 | ENST00000424699.5 | 1616 | 105aa | Nonsense mediated decay | - | G5E9Q4 | - | TSL:2, |
| PRKRA-226 | ENST00000677859.1 | 1600 | 94aa | Nonsense mediated decay | - | - | - | CDS 5' incomplete, |
| PRKRA-230 | ENST00000678167.1 | 1587 | 106aa | Nonsense mediated decay | - | - | - | - |
| PRKRA-229 | ENST00000678058.1 | 1343 | 56aa | Nonsense mediated decay | - | - | - | CDS 5' incomplete, |
| PRKRA-209 | ENST00000470200.5 | 550 | No protein | Processed transcript | - | - | - | TSL:4, |
| PRKRA-206 | ENST00000460433.5 | 461 | No protein | Processed transcript | - | - | - | TSL:3, |
| PRKRA-225 | ENST00000677806.1 | 3890 | No protein | Retained intron | - | - | - | - |
| PRKRA-214 | ENST00000676586.1 | 3741 | No protein | Retained intron | - | - | - | - |
| PRKRA-218 | ENST00000677136.1 | 3596 | No protein | Retained intron | - | - | - | - |
| PRKRA-215 | ENST00000676752.1 | 3498 | No protein | Retained intron | - | - | - | - |
| PRKRA-236 | ENST00000679202.1 | 2686 | No protein | Retained intron | - | - | - | - |
| PRKRA-233 | ENST00000678813.1 | 2209 | No protein | Retained intron | - | - | - | - |
| PRKRA-212 | ENST00000490501.5 | 1851 | No protein | Retained intron | - | - | - | TSL:2, |
| PRKRA-210 | ENST00000474793.6 | 1745 | No protein | Retained intron | - | - | - | TSL:3, |
| PRKRA-231 | ENST00000678379.1 | 1621 | No protein | Retained intron | - | - | - | - |
| PRKRA-208 | ENST00000466165.1 | 585 | No protein | Retained intron | - | - | - | TSL:2, |
| PRKRA-207 | ENST00000463882.1 | 541 | No protein | Retained intron | - | - | - | TSL:2, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | PRKRA |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene PRKRA had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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