CRISPR-U™ Gene Knockout Cell Line Strategy

MKNK1 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human MKNK1 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol MKNK1
Gene id 8569
Organism Homo sapiens
Official full symbol MAPK interacting serine/threonine kinase 1
Gene type protein-coding
Also known as MNK1
Summary This gene encodes a Ser/Thr protein kinase that interacts with, and is activated by ERK1 and p38 mitogen-activated protein kinases, and thus may play a role in the response to environmental stress and cytokines. This kinase may also regulate transcription by phosphorylating eIF4E via interaction with the C-terminal region of eIF4G. Alternatively spliced transcript variants have been noted for this gene.
Genomic regions Chromosome 1
Strategy Summary
This gene has 14 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
MKNK1-229 ENST00000649800.1 2809 465aa Protein coding CCDS538 Q9BUB5 - GENCODE basic,
MKNK1-231 ENST00000650508.1 2686 424aa Protein coding CCDS44134 - - GENCODE basic, APPRIS ALT2,
MKNK1-230 ENST00000650026.1 2600 347aa Protein coding CCDS30705 - - GENCODE basic,
MKNK1-204 ENST00000371946.9 2719 453aa Protein coding - A0A499FIS5 - TSL:1, GENCODE basic,
MKNK1-203 ENST00000371945.10 2661 412aa Protein coding - A0A499FJN1 NM_001135553.4 TSL:1, GENCODE basic, APPRIS P2, MANE Select v0.92,
MKNK1-201 ENST00000341183.9 2612 329aa Protein coding - Q7Z319 - TSL:5, GENCODE basic,
MKNK1-205 ENST00000428112.7 1270 335aa Protein coding - A0A499FJS1 - TSL:2, GENCODE basic,
MKNK1-217 ENST00000524749.5 786 85aa Protein coding - H0YE67 - CDS 5' incomplete, TSL:5,
MKNK1-215 ENST00000496619.6 738 166aa Protein coding - E9PIA0 - CDS 3' incomplete, TSL:3,
MKNK1-225 ENST00000531769.6 567 88aa Protein coding - E9PSE0 - CDS 3' incomplete, TSL:4,
MKNK1-210 ENST00000474868.2 540 79aa Protein coding - E9PN34 - TSL:3, GENCODE basic,
MKNK1-222 ENST00000529170.6 530 89aa Protein coding - E9PLE7 - CDS 3' incomplete, TSL:4,
MKNK1-226 ENST00000532110.1 494 126aa Protein coding - E9PII5 - CDS 3' incomplete, TSL:4,
MKNK1-207 ENST00000465783.6 464 79aa Protein coding - E9PN34 - TSL:2, GENCODE basic,
MKNK1-220 ENST00000528077.6 1557 106aa Nonsense mediated decay - E9PMM8 - TSL:2,
MKNK1-224 ENST00000531355.6 1121 44aa Nonsense mediated decay - E9PQ26 - TSL:1,
MKNK1-219 ENST00000526513.6 682 48aa Nonsense mediated decay - E9PSC9 - TSL:5,
MKNK1-202 ENST00000342571.7 2422 No protein Processed transcript - - - TSL:1,
MKNK1-218 ENST00000525888.5 588 No protein Processed transcript - - - TSL:4,
MKNK1-227 ENST00000532783.5 580 No protein Processed transcript - - - TSL:4,
MKNK1-209 ENST00000470237.5 562 No protein Processed transcript - - - TSL:3,
MKNK1-221 ENST00000528237.5 540 No protein Processed transcript - - - TSL:4,
MKNK1-206 ENST00000460098.1 337 No protein Processed transcript - - - TSL:3,
MKNK1-213 ENST00000484301.5 3408 No protein Retained intron - - - TSL:1,
MKNK1-228 ENST00000532897.5 795 No protein Retained intron - - - TSL:2,
MKNK1-211 ENST00000477883.1 696 No protein Retained intron - - - TSL:3,
MKNK1-212 ENST00000480531.5 649 No protein Retained intron - - - TSL:3,
MKNK1-214 ENST00000486716.5 594 No protein Retained intron - - - TSL:2,
MKNK1-208 ENST00000468852.2 584 No protein Retained intron - - - TSL:3,
MKNK1-223 ENST00000530528.1 553 No protein Retained intron - - - TSL:2,
MKNK1-216 ENST00000524417.1 531 No protein Retained intron - - - TSL:4,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene MKNK1
This KO Strategy loading
Red Cotton™ Notes Gene MKNK1 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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