CRISPR-U™ Gene Knockout Cell Line
Strategy
MKNK1 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human MKNK1 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | MKNK1 |
| Gene id | 8569 |
| Organism | Homo sapiens |
| Official full symbol | MAPK interacting serine/threonine kinase 1 |
| Gene type | protein-coding |
| Also known as | MNK1 |
| Summary | This gene encodes a Ser/Thr protein kinase that interacts with, and is activated by ERK1 and p38 mitogen-activated protein kinases, and thus may play a role in the response to environmental stress and cytokines. This kinase may also regulate transcription by phosphorylating eIF4E via interaction with the C-terminal region of eIF4G. Alternatively spliced transcript variants have been noted for this gene. |
| Genomic regions | Chromosome 1 |
Strategy Summary
This gene has 14 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| MKNK1-229 | ENST00000649800.1 | 2809 | 465aa | Protein coding | CCDS538 | Q9BUB5 | - | GENCODE basic, |
| MKNK1-231 | ENST00000650508.1 | 2686 | 424aa | Protein coding | CCDS44134 | - | - | GENCODE basic, APPRIS ALT2, |
| MKNK1-230 | ENST00000650026.1 | 2600 | 347aa | Protein coding | CCDS30705 | - | - | GENCODE basic, |
| MKNK1-204 | ENST00000371946.9 | 2719 | 453aa | Protein coding | - | A0A499FIS5 | - | TSL:1, GENCODE basic, |
| MKNK1-203 | ENST00000371945.10 | 2661 | 412aa | Protein coding | - | A0A499FJN1 | NM_001135553.4 | TSL:1, GENCODE basic, APPRIS P2, MANE Select v0.92, |
| MKNK1-201 | ENST00000341183.9 | 2612 | 329aa | Protein coding | - | Q7Z319 | - | TSL:5, GENCODE basic, |
| MKNK1-205 | ENST00000428112.7 | 1270 | 335aa | Protein coding | - | A0A499FJS1 | - | TSL:2, GENCODE basic, |
| MKNK1-217 | ENST00000524749.5 | 786 | 85aa | Protein coding | - | H0YE67 | - | CDS 5' incomplete, TSL:5, |
| MKNK1-215 | ENST00000496619.6 | 738 | 166aa | Protein coding | - | E9PIA0 | - | CDS 3' incomplete, TSL:3, |
| MKNK1-225 | ENST00000531769.6 | 567 | 88aa | Protein coding | - | E9PSE0 | - | CDS 3' incomplete, TSL:4, |
| MKNK1-210 | ENST00000474868.2 | 540 | 79aa | Protein coding | - | E9PN34 | - | TSL:3, GENCODE basic, |
| MKNK1-222 | ENST00000529170.6 | 530 | 89aa | Protein coding | - | E9PLE7 | - | CDS 3' incomplete, TSL:4, |
| MKNK1-226 | ENST00000532110.1 | 494 | 126aa | Protein coding | - | E9PII5 | - | CDS 3' incomplete, TSL:4, |
| MKNK1-207 | ENST00000465783.6 | 464 | 79aa | Protein coding | - | E9PN34 | - | TSL:2, GENCODE basic, |
| MKNK1-220 | ENST00000528077.6 | 1557 | 106aa | Nonsense mediated decay | - | E9PMM8 | - | TSL:2, |
| MKNK1-224 | ENST00000531355.6 | 1121 | 44aa | Nonsense mediated decay | - | E9PQ26 | - | TSL:1, |
| MKNK1-219 | ENST00000526513.6 | 682 | 48aa | Nonsense mediated decay | - | E9PSC9 | - | TSL:5, |
| MKNK1-202 | ENST00000342571.7 | 2422 | No protein | Processed transcript | - | - | - | TSL:1, |
| MKNK1-218 | ENST00000525888.5 | 588 | No protein | Processed transcript | - | - | - | TSL:4, |
| MKNK1-227 | ENST00000532783.5 | 580 | No protein | Processed transcript | - | - | - | TSL:4, |
| MKNK1-209 | ENST00000470237.5 | 562 | No protein | Processed transcript | - | - | - | TSL:3, |
| MKNK1-221 | ENST00000528237.5 | 540 | No protein | Processed transcript | - | - | - | TSL:4, |
| MKNK1-206 | ENST00000460098.1 | 337 | No protein | Processed transcript | - | - | - | TSL:3, |
| MKNK1-213 | ENST00000484301.5 | 3408 | No protein | Retained intron | - | - | - | TSL:1, |
| MKNK1-228 | ENST00000532897.5 | 795 | No protein | Retained intron | - | - | - | TSL:2, |
| MKNK1-211 | ENST00000477883.1 | 696 | No protein | Retained intron | - | - | - | TSL:3, |
| MKNK1-212 | ENST00000480531.5 | 649 | No protein | Retained intron | - | - | - | TSL:3, |
| MKNK1-214 | ENST00000486716.5 | 594 | No protein | Retained intron | - | - | - | TSL:2, |
| MKNK1-208 | ENST00000468852.2 | 584 | No protein | Retained intron | - | - | - | TSL:3, |
| MKNK1-223 | ENST00000530528.1 | 553 | No protein | Retained intron | - | - | - | TSL:2, |
| MKNK1-216 | ENST00000524417.1 | 531 | No protein | Retained intron | - | - | - | TSL:4, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | MKNK1 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene MKNK1 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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