CRISPR-U™ Gene Knockout Cell Line Strategy

PDXK Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human PDXK Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol PDXK
Gene id 8566
Organism Homo sapiens
Official full symbol pyridoxal kinase
Gene type protein-coding
Also known as C21orf124, C21orf97, HEL-S-1a, HMSN6C, PKH, PNK, PRED79
Summary The protein encoded by this gene phosphorylates vitamin B6, a step required for the conversion of vitamin B6 to pyridoxal-5-phosphate, an important cofactor in intermediary metabolism. The encoded protein is cytoplasmic and probably acts as a homodimer. Alternatively spliced transcript variants have been described, but their biological validity has not been determined.
Genomic regions Chromosome 21
Strategy Summary
This gene has 6 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
PDXK-201 ENST00000291565.9 7341 312aa Protein coding CCDS13699 O00764-1 NM_003681.5 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
PDXK-209 ENST00000467908.1 7194 272aa Protein coding CCDS82679 F2Z2Y4 - TSL:3, GENCODE basic,
PDXK-210 ENST00000468090.5 7297 284aa Protein coding - O00764-2 - TSL:1, GENCODE basic,
PDXK-202 ENST00000327574.4 2583 148aa Protein coding - A8MT14 - TSL:2, GENCODE basic,
PDXK-205 ENST00000398081.5 2251 87aa Protein coding - A8MV33 - TSL:2, GENCODE basic,
PDXK-219 ENST00000621478.1 1967 162aa Protein coding - A0A0B4J2C9 - TSL:1, GENCODE basic,
PDXK-215 ENST00000476313.5 1704 No protein Processed transcript - - - TSL:3,
PDXK-203 ENST00000343528.10 1101 No protein Processed transcript - - - TSL:1,
PDXK-211 ENST00000468392.5 1094 No protein Processed transcript - - - TSL:2,
PDXK-204 ENST00000398078.7 1069 No protein Processed transcript - - - TSL:2,
PDXK-218 ENST00000498040.5 888 No protein Processed transcript - - - TSL:5,
PDXK-206 ENST00000398085.7 796 No protein Processed transcript - - - TSL:3,
PDXK-208 ENST00000461123.1 637 No protein Processed transcript - - - TSL:3,
PDXK-214 ENST00000476084.1 602 No protein Processed transcript - - - TSL:3,
PDXK-212 ENST00000470029.5 583 No protein Processed transcript - - - TSL:3,
PDXK-216 ENST00000481512.5 583 No protein Processed transcript - - - TSL:5,
PDXK-217 ENST00000490666.5 577 No protein Processed transcript - - - TSL:5,
PDXK-213 ENST00000472777.5 460 No protein Processed transcript - - - TSL:5,
PDXK-207 ENST00000438837.1 342 No protein Processed transcript - - - TSL:3,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene PDXK
This KO Strategy loading
Red Cotton™ Notes Gene PDXK had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

Please leave your suggestion ×