CRISPR-U™ Gene Knockout Cell Line
Strategy
PDXK Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human PDXK Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | PDXK |
| Gene id | 8566 |
| Organism | Homo sapiens |
| Official full symbol | pyridoxal kinase |
| Gene type | protein-coding |
| Also known as | C21orf124, C21orf97, HEL-S-1a, HMSN6C, PKH, PNK, PRED79 |
| Summary | The protein encoded by this gene phosphorylates vitamin B6, a step required for the conversion of vitamin B6 to pyridoxal-5-phosphate, an important cofactor in intermediary metabolism. The encoded protein is cytoplasmic and probably acts as a homodimer. Alternatively spliced transcript variants have been described, but their biological validity has not been determined. |
| Genomic regions | Chromosome 21 |
Strategy Summary
This gene has 6 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| PDXK-201 | ENST00000291565.9 | 7341 | 312aa | Protein coding | CCDS13699 | O00764-1 | NM_003681.5 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| PDXK-209 | ENST00000467908.1 | 7194 | 272aa | Protein coding | CCDS82679 | F2Z2Y4 | - | TSL:3, GENCODE basic, |
| PDXK-210 | ENST00000468090.5 | 7297 | 284aa | Protein coding | - | O00764-2 | - | TSL:1, GENCODE basic, |
| PDXK-202 | ENST00000327574.4 | 2583 | 148aa | Protein coding | - | A8MT14 | - | TSL:2, GENCODE basic, |
| PDXK-205 | ENST00000398081.5 | 2251 | 87aa | Protein coding | - | A8MV33 | - | TSL:2, GENCODE basic, |
| PDXK-219 | ENST00000621478.1 | 1967 | 162aa | Protein coding | - | A0A0B4J2C9 | - | TSL:1, GENCODE basic, |
| PDXK-215 | ENST00000476313.5 | 1704 | No protein | Processed transcript | - | - | - | TSL:3, |
| PDXK-203 | ENST00000343528.10 | 1101 | No protein | Processed transcript | - | - | - | TSL:1, |
| PDXK-211 | ENST00000468392.5 | 1094 | No protein | Processed transcript | - | - | - | TSL:2, |
| PDXK-204 | ENST00000398078.7 | 1069 | No protein | Processed transcript | - | - | - | TSL:2, |
| PDXK-218 | ENST00000498040.5 | 888 | No protein | Processed transcript | - | - | - | TSL:5, |
| PDXK-206 | ENST00000398085.7 | 796 | No protein | Processed transcript | - | - | - | TSL:3, |
| PDXK-208 | ENST00000461123.1 | 637 | No protein | Processed transcript | - | - | - | TSL:3, |
| PDXK-214 | ENST00000476084.1 | 602 | No protein | Processed transcript | - | - | - | TSL:3, |
| PDXK-212 | ENST00000470029.5 | 583 | No protein | Processed transcript | - | - | - | TSL:3, |
| PDXK-216 | ENST00000481512.5 | 583 | No protein | Processed transcript | - | - | - | TSL:5, |
| PDXK-217 | ENST00000490666.5 | 577 | No protein | Processed transcript | - | - | - | TSL:5, |
| PDXK-213 | ENST00000472777.5 | 460 | No protein | Processed transcript | - | - | - | TSL:5, |
| PDXK-207 | ENST00000438837.1 | 342 | No protein | Processed transcript | - | - | - | TSL:3, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | PDXK |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene PDXK had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
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Work flow
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