CRISPR-U™ Gene Knockout Cell Line
Strategy
CDC14B Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human CDC14B Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | CDC14B |
| Gene id | 8555 |
| Organism | Homo sapiens |
| Official full symbol | cell division cycle 14B |
| Gene type | protein-coding |
| Also known as | CDC14B3, Cdc14B1, Cdc14B2, hCDC14B |
| Summary | The protein encoded by this gene is a member of the dual specificity protein tyrosine phosphatase family. This protein is highly similar to Saccharomyces cerevisiae Cdc14, a protein tyrosine phosphatase involved in the exit of cell mitosis and initiation of DNA replication, which suggests the role in cell cycle control. This protein has been shown to interact with and dephosphorylates tumor suppressor protein p53, and is thought to regulate the function of p53. Alternative splice of this gene results in 3 transcript variants encoding distinct isoforms. |
| Genomic regions | Chromosome 9 |
Strategy Summary
This gene has 6 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| CDC14B-202 | ENST00000375241.6 | 5602 | 498aa | Protein coding | CCDS6722 | O60729-1 | NM_033331.4 | TSL:1, GENCODE basic, APPRIS P4, MANE Select v0.92, |
| CDC14B-203 | ENST00000375242.7 | 2965 | 461aa | Protein coding | CCDS43853 | O60729-5 | - | TSL:2, GENCODE basic, APPRIS ALT1, |
| CDC14B-201 | ENST00000375240.7 | 2547 | 459aa | Protein coding | CCDS6721 | O60729-2 | - | TSL:1, GENCODE basic, APPRIS ALT2, |
| CDC14B-208 | ENST00000463569.5 | 1734 | 485aa | Protein coding | CCDS87662 | O60729-4 | - | TSL:5, GENCODE basic, APPRIS ALT2, |
| CDC14B-206 | ENST00000452280.5 | 804 | 268aa | Protein coding | - | H0Y577 | - | CDS 5' and 3' incomplete, TSL:3, |
| CDC14B-205 | ENST00000415608.1 | 684 | 228aa | Protein coding | - | Q5JU07 | - | CDS 5' and 3' incomplete, TSL:5, |
| CDC14B-204 | ENST00000412285.6 | 5271 | 480aa | Nonsense mediated decay | - | H7C3U8 | - | CDS 5' incomplete, TSL:1, |
| CDC14B-209 | ENST00000474602.5 | 4960 | 471aa | Nonsense mediated decay | - | O60729-3 | - | TSL:1, |
| CDC14B-211 | ENST00000481149.1 | 689 | No protein | Processed transcript | - | - | - | TSL:3, |
| CDC14B-207 | ENST00000463547.5 | 553 | No protein | Processed transcript | - | - | - | TSL:4, |
| CDC14B-210 | ENST00000480920.5 | 459 | No protein | Processed transcript | - | - | - | TSL:3, |
| CDC14B-213 | ENST00000496750.5 | 649 | No protein | Retained intron | - | - | - | TSL:3, |
| CDC14B-212 | ENST00000484948.1 | 350 | No protein | Retained intron | - | - | - | TSL:5, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | CDC14B |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene CDC14B had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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