CRISPR-U™ Gene Knockout Cell Line Strategy

CDC14B Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human CDC14B Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol CDC14B
Gene id 8555
Organism Homo sapiens
Official full symbol cell division cycle 14B
Gene type protein-coding
Also known as CDC14B3, Cdc14B1, Cdc14B2, hCDC14B
Summary The protein encoded by this gene is a member of the dual specificity protein tyrosine phosphatase family. This protein is highly similar to Saccharomyces cerevisiae Cdc14, a protein tyrosine phosphatase involved in the exit of cell mitosis and initiation of DNA replication, which suggests the role in cell cycle control. This protein has been shown to interact with and dephosphorylates tumor suppressor protein p53, and is thought to regulate the function of p53. Alternative splice of this gene results in 3 transcript variants encoding distinct isoforms.
Genomic regions Chromosome 9
Strategy Summary
This gene has 6 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
CDC14B-202 ENST00000375241.6 5602 498aa Protein coding CCDS6722 O60729-1 NM_033331.4 TSL:1, GENCODE basic, APPRIS P4, MANE Select v0.92,
CDC14B-203 ENST00000375242.7 2965 461aa Protein coding CCDS43853 O60729-5 - TSL:2, GENCODE basic, APPRIS ALT1,
CDC14B-201 ENST00000375240.7 2547 459aa Protein coding CCDS6721 O60729-2 - TSL:1, GENCODE basic, APPRIS ALT2,
CDC14B-208 ENST00000463569.5 1734 485aa Protein coding CCDS87662 O60729-4 - TSL:5, GENCODE basic, APPRIS ALT2,
CDC14B-206 ENST00000452280.5 804 268aa Protein coding - H0Y577 - CDS 5' and 3' incomplete, TSL:3,
CDC14B-205 ENST00000415608.1 684 228aa Protein coding - Q5JU07 - CDS 5' and 3' incomplete, TSL:5,
CDC14B-204 ENST00000412285.6 5271 480aa Nonsense mediated decay - H7C3U8 - CDS 5' incomplete, TSL:1,
CDC14B-209 ENST00000474602.5 4960 471aa Nonsense mediated decay - O60729-3 - TSL:1,
CDC14B-211 ENST00000481149.1 689 No protein Processed transcript - - - TSL:3,
CDC14B-207 ENST00000463547.5 553 No protein Processed transcript - - - TSL:4,
CDC14B-210 ENST00000480920.5 459 No protein Processed transcript - - - TSL:3,
CDC14B-213 ENST00000496750.5 649 No protein Retained intron - - - TSL:3,
CDC14B-212 ENST00000484948.1 350 No protein Retained intron - - - TSL:5,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene CDC14B
This KO Strategy loading
Red Cotton™ Notes Gene CDC14B had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
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Work flow
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