CRISPR-U™ Gene Knockout Cell Line Strategy

MAPKAPK5 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human MAPKAPK5 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol MAPKAPK5
Gene id 8550
Organism Homo sapiens
Official full symbol MAPK activated protein kinase 5
Gene type protein-coding
Also known as MAPKAP-K5, MK-5, MK5, PRAK
Summary The protein encoded by this gene is a tumor suppressor and member of the serine/threonine kinase family. In response to cellular stress and proinflammatory cytokines, this kinase is activated through its phosphorylation by MAP kinases including MAPK1/ERK, MAPK14/p38-alpha, and MAPK11/p38-beta. The encoded protein is found in the nucleus but translocates to the cytoplasm upon phosphorylation and activation. This kinase phosphorylates heat shock protein HSP27 at its physiologically relevant sites. Two alternately spliced transcript variants of this gene encoding distinct isoforms have been reported.
Genomic regions Chromosome 12
Strategy Summary
This gene has 4 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
MAPKAPK5-206 ENST00000550735.7 11083 471aa Protein coding CCDS44976 Q8IW41-2 NM_003668.4 TSL:1, GENCODE basic, APPRIS P4, MANE Select v0.92,
MAPKAPK5-207 ENST00000551404.6 1630 473aa Protein coding CCDS44975 Q8IW41-1 - TSL:5, GENCODE basic, APPRIS ALT1,
MAPKAPK5-205 ENST00000549875.1 974 324aa Protein coding - R4GN33 - CDS 3' incomplete, TSL:5,
MAPKAPK5-203 ENST00000547305.1 494 51aa Protein coding - R4GN93 - TSL:2, GENCODE basic,
MAPKAPK5-209 ENST00000553053.5 875 74aa Nonsense mediated decay - F8VRP2 - TSL:5,
MAPKAPK5-201 ENST00000546394.1 567 No protein Processed transcript - - - TSL:3,
MAPKAPK5-202 ENST00000547067.1 1459 No protein Retained intron - - - TSL:2,
MAPKAPK5-204 ENST00000547915.1 1360 No protein Retained intron - - - TSL:3,
MAPKAPK5-208 ENST00000552111.5 954 No protein Retained intron - - - TSL:3,
MAPKAPK5-210 ENST00000602983.1 392 No protein Retained intron - - - TSL:NA,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene MAPKAPK5
This KO Strategy loading
Red Cotton™ Notes Gene MAPKAPK5 had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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