CRISPR-U™ Gene Knockout Cell Line
Strategy
MAPKAPK5 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human MAPKAPK5 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | MAPKAPK5 |
| Gene id | 8550 |
| Organism | Homo sapiens |
| Official full symbol | MAPK activated protein kinase 5 |
| Gene type | protein-coding |
| Also known as | MAPKAP-K5, MK-5, MK5, PRAK |
| Summary | The protein encoded by this gene is a tumor suppressor and member of the serine/threonine kinase family. In response to cellular stress and proinflammatory cytokines, this kinase is activated through its phosphorylation by MAP kinases including MAPK1/ERK, MAPK14/p38-alpha, and MAPK11/p38-beta. The encoded protein is found in the nucleus but translocates to the cytoplasm upon phosphorylation and activation. This kinase phosphorylates heat shock protein HSP27 at its physiologically relevant sites. Two alternately spliced transcript variants of this gene encoding distinct isoforms have been reported. |
| Genomic regions | Chromosome 12 |
Strategy Summary
This gene has 4 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| MAPKAPK5-206 | ENST00000550735.7 | 11083 | 471aa | Protein coding | CCDS44976 | Q8IW41-2 | NM_003668.4 | TSL:1, GENCODE basic, APPRIS P4, MANE Select v0.92, |
| MAPKAPK5-207 | ENST00000551404.6 | 1630 | 473aa | Protein coding | CCDS44975 | Q8IW41-1 | - | TSL:5, GENCODE basic, APPRIS ALT1, |
| MAPKAPK5-205 | ENST00000549875.1 | 974 | 324aa | Protein coding | - | R4GN33 | - | CDS 3' incomplete, TSL:5, |
| MAPKAPK5-203 | ENST00000547305.1 | 494 | 51aa | Protein coding | - | R4GN93 | - | TSL:2, GENCODE basic, |
| MAPKAPK5-209 | ENST00000553053.5 | 875 | 74aa | Nonsense mediated decay | - | F8VRP2 | - | TSL:5, |
| MAPKAPK5-201 | ENST00000546394.1 | 567 | No protein | Processed transcript | - | - | - | TSL:3, |
| MAPKAPK5-202 | ENST00000547067.1 | 1459 | No protein | Retained intron | - | - | - | TSL:2, |
| MAPKAPK5-204 | ENST00000547915.1 | 1360 | No protein | Retained intron | - | - | - | TSL:3, |
| MAPKAPK5-208 | ENST00000552111.5 | 954 | No protein | Retained intron | - | - | - | TSL:3, |
| MAPKAPK5-210 | ENST00000602983.1 | 392 | No protein | Retained intron | - | - | - | TSL:NA, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | MAPKAPK5 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene MAPKAPK5 had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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