CRISPR-U™ Gene Knockout Cell Line
Strategy
LGR5 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human LGR5 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | LGR5 |
| Gene id | 8549 |
| Organism | Homo sapiens |
| Official full symbol | leucine rich repeat containing G protein-coupled receptor 5 |
| Gene type | protein-coding |
| Also known as | FEX, GPR49, GPR67, GRP49, HG38 |
| Summary | The protein encoded by this gene is a leucine-rich repeat-containing receptor (LGR) and member of the G protein-coupled, 7-transmembrane receptor (GPCR) superfamily. The encoded protein is a receptor for R-spondins and is involved in the canonical Wnt signaling pathway. This protein plays a role in the formation and maintenance of adult intestinal stem cells during postembryonic development. Several transcript variants encoding different isoforms have been found for this gene. |
| Genomic regions | Chromosome 12 |
Strategy Summary
This gene has 3 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| LGR5-201 | ENST00000266674.10 | 4583 | 907aa | Protein coding | CCDS9000 | O75473-1 | NM_003667.4 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| LGR5-203 | ENST00000540815.2 | 2800 | 883aa | Protein coding | CCDS61194 | O75473-2 | - | TSL:1, GENCODE basic, |
| LGR5-202 | ENST00000536515.5 | 2664 | 835aa | Protein coding | CCDS61195 | O75473-3 | - | TSL:1, GENCODE basic, |
| LGR5-206 | ENST00000550851.5 | 4095 | No protein | Retained intron | - | - | - | TSL:2, |
| LGR5-204 | ENST00000547310.1 | 576 | No protein | Retained intron | - | - | - | TSL:3, |
| LGR5-205 | ENST00000549015.1 | 298 | No protein | Retained intron | - | - | - | TSL:3, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | LGR5 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene LGR5 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
Comment: