CRISPR-U™ Gene Knockout Cell Line Strategy

LGR5 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human LGR5 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol LGR5
Gene id 8549
Organism Homo sapiens
Official full symbol leucine rich repeat containing G protein-coupled receptor 5
Gene type protein-coding
Also known as FEX, GPR49, GPR67, GRP49, HG38
Summary The protein encoded by this gene is a leucine-rich repeat-containing receptor (LGR) and member of the G protein-coupled, 7-transmembrane receptor (GPCR) superfamily. The encoded protein is a receptor for R-spondins and is involved in the canonical Wnt signaling pathway. This protein plays a role in the formation and maintenance of adult intestinal stem cells during postembryonic development. Several transcript variants encoding different isoforms have been found for this gene.
Genomic regions Chromosome 12
Strategy Summary
This gene has 3 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
LGR5-201 ENST00000266674.10 4583 907aa Protein coding CCDS9000 O75473-1 NM_003667.4 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
LGR5-203 ENST00000540815.2 2800 883aa Protein coding CCDS61194 O75473-2 - TSL:1, GENCODE basic,
LGR5-202 ENST00000536515.5 2664 835aa Protein coding CCDS61195 O75473-3 - TSL:1, GENCODE basic,
LGR5-206 ENST00000550851.5 4095 No protein Retained intron - - - TSL:2,
LGR5-204 ENST00000547310.1 576 No protein Retained intron - - - TSL:3,
LGR5-205 ENST00000549015.1 298 No protein Retained intron - - - TSL:3,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene LGR5
This KO Strategy loading
Red Cotton™ Notes Gene LGR5 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:

$49

Single gRNA plasmid off-shelf

$599

Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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