CRISPR-U™ Gene Knockout Cell Line Strategy

HELZ2 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human HELZ2 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol HELZ2
Gene id 85441
Organism Homo sapiens
Official full symbol helicase with zinc finger 2
Gene type protein-coding
Also known as PDIP-1, PRIC285
Summary The protein encoded by this gene is a nuclear transcriptional co-activator for peroxisome proliferator activated receptor alpha. The encoded protein contains a zinc finger and is a helicase that appears to be part of the peroxisome proliferator activated receptor alpha interacting complex. This gene is a member of the DNA2/NAM7 helicase gene family. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Genomic regions Chromosome 20
Strategy Summary
This gene has 2 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
HELZ2-204 ENST00000467148.1 8064 2649aa Protein coding CCDS33508 Q9BYK8-1 - TSL:1, GENCODE basic, APPRIS P1,
HELZ2-202 ENST00000427522.6 7827 2080aa Protein coding CCDS13527 Q9BYK8-2 - TSL:1, GENCODE basic,
HELZ2-206 ENST00000479540.5 3079 No protein Processed transcript - - - TSL:1,
HELZ2-205 ENST00000478861.1 833 No protein Processed transcript - - - TSL:5,
HELZ2-203 ENST00000454223.1 435 No protein Processed transcript - - - TSL:2,
HELZ2-201 ENST00000370082.1 1827 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene HELZ2
This KO Strategy loading
Red Cotton™ Notes Gene HELZ2 had been KO in a549 cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
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Work flow
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