CRISPR-U™ Gene Knockout Cell Line Strategy

PPP1R16A Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human PPP1R16A Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol PPP1R16A
Gene id 84988
Organism Homo sapiens
Official full symbol protein phosphatase 1 regulatory subunit 16A
Gene type protein-coding
Also known as MYPT3
Summary Myosin light chain kinase and phosphatase (MLCP) complexes control the phosphorylation states of regulatory myosin light chains, which is crucial for muscle and intracellular movement. MLCPs typically contain a catalytic protein phosphatase 1 (PP1c) subunit, a myosin phosphatase targeting (MYPT) subunit, and another smaller subunit. The protein encoded by this gene represents an MYPT subunit, which is responsible for directing PP1c to its intended targets. However, while the phosphorylation of other MYPT members results in PP1c inactivation, phosphorylation of the encoded protein by protein kinase A results in PP1c activation.
Genomic regions Chromosome 8
Strategy Summary
This gene has 3 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
PPP1R16A-201 ENST00000292539.8 2722 528aa Protein coding CCDS6429 Q96I34 - TSL:2, GENCODE basic, APPRIS P1,
PPP1R16A-202 ENST00000435887.1 2273 528aa Protein coding CCDS6429 Q96I34 - TSL:1, GENCODE basic, APPRIS P1,
PPP1R16A-207 ENST00000529009.1 657 65aa Protein coding - A0A087WWZ0 - CDS 3' incomplete, TSL:3,
PPP1R16A-208 ENST00000529283.5 636 No protein Processed transcript - - - TSL:5,
PPP1R16A-210 ENST00000533088.1 577 No protein Processed transcript - - - TSL:2,
PPP1R16A-211 ENST00000533829.1 558 No protein Processed transcript - - - TSL:3,
PPP1R16A-203 ENST00000526183.5 4311 No protein Retained intron - - - TSL:2,
PPP1R16A-206 ENST00000528430.2 807 No protein Retained intron - - - TSL:2,
PPP1R16A-209 ENST00000532806.1 747 No protein Retained intron - - - TSL:5,
PPP1R16A-204 ENST00000526564.1 675 No protein Retained intron - - - TSL:5,
PPP1R16A-205 ENST00000526643.1 663 No protein Retained intron - - - TSL:4,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene PPP1R16A
This KO Strategy loading
Red Cotton™ Notes Gene PPP1R16A had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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