CRISPR-U™ Gene Knockout Cell Line
Strategy
PPP1R16A Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human PPP1R16A Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | PPP1R16A |
| Gene id | 84988 |
| Organism | Homo sapiens |
| Official full symbol | protein phosphatase 1 regulatory subunit 16A |
| Gene type | protein-coding |
| Also known as | MYPT3 |
| Summary | Myosin light chain kinase and phosphatase (MLCP) complexes control the phosphorylation states of regulatory myosin light chains, which is crucial for muscle and intracellular movement. MLCPs typically contain a catalytic protein phosphatase 1 (PP1c) subunit, a myosin phosphatase targeting (MYPT) subunit, and another smaller subunit. The protein encoded by this gene represents an MYPT subunit, which is responsible for directing PP1c to its intended targets. However, while the phosphorylation of other MYPT members results in PP1c inactivation, phosphorylation of the encoded protein by protein kinase A results in PP1c activation. |
| Genomic regions | Chromosome 8 |
Strategy Summary
This gene has 3 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| PPP1R16A-201 | ENST00000292539.8 | 2722 | 528aa | Protein coding | CCDS6429 | Q96I34 | - | TSL:2, GENCODE basic, APPRIS P1, |
| PPP1R16A-202 | ENST00000435887.1 | 2273 | 528aa | Protein coding | CCDS6429 | Q96I34 | - | TSL:1, GENCODE basic, APPRIS P1, |
| PPP1R16A-207 | ENST00000529009.1 | 657 | 65aa | Protein coding | - | A0A087WWZ0 | - | CDS 3' incomplete, TSL:3, |
| PPP1R16A-208 | ENST00000529283.5 | 636 | No protein | Processed transcript | - | - | - | TSL:5, |
| PPP1R16A-210 | ENST00000533088.1 | 577 | No protein | Processed transcript | - | - | - | TSL:2, |
| PPP1R16A-211 | ENST00000533829.1 | 558 | No protein | Processed transcript | - | - | - | TSL:3, |
| PPP1R16A-203 | ENST00000526183.5 | 4311 | No protein | Retained intron | - | - | - | TSL:2, |
| PPP1R16A-206 | ENST00000528430.2 | 807 | No protein | Retained intron | - | - | - | TSL:2, |
| PPP1R16A-209 | ENST00000532806.1 | 747 | No protein | Retained intron | - | - | - | TSL:5, |
| PPP1R16A-204 | ENST00000526564.1 | 675 | No protein | Retained intron | - | - | - | TSL:5, |
| PPP1R16A-205 | ENST00000526643.1 | 663 | No protein | Retained intron | - | - | - | TSL:4, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | PPP1R16A |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene PPP1R16A had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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