CRISPR-U™ Gene Knockout Cell Line
Strategy
MPND Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human MPND Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | MPND |
| Gene id | 84954 |
| Organism | Homo sapiens |
| Official full symbol | MPN domain containing |
| Gene type | protein-coding |
| Genomic regions | Chromosome 19 |
Strategy Summary
This gene has 7 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| MPND-207 | ENST00000599840.6 | 1614 | 501aa | Protein coding | CCDS74261 | W4VSR2 | NM_001300862.2 | TSL:5, GENCODE basic, APPRIS P2, MANE Select v0.92, |
| MPND-201 | ENST00000262966.12 | 1567 | 471aa | Protein coding | CCDS42470 | Q8N594-1 | - | TSL:1, GENCODE basic, APPRIS ALT2, |
| MPND-202 | ENST00000359935.8 | 1475 | 451aa | Protein coding | CCDS54200 | Q8N594-2 | - | TSL:2, GENCODE basic, |
| MPND-205 | ENST00000596722.5 | 1578 | 503aa | Protein coding | - | M0R189 | - | CDS 5' incomplete, TSL:2, |
| MPND-206 | ENST00000597036.5 | 1445 | 469aa | Protein coding | - | M0R0F2 | - | CDS 5' incomplete, TSL:5, |
| MPND-203 | ENST00000594162.1 | 407 | 109aa | Protein coding | - | M0QXC4 | - | CDS 5' incomplete, TSL:3, |
| MPND-208 | ENST00000601877.1 | 379 | 121aa | Protein coding | - | M0QXK5 | - | CDS 5' incomplete, TSL:5, |
| MPND-204 | ENST00000594716.5 | 1547 | 419aa | Nonsense mediated decay | - | M0R044 | - | TSL:1, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | MPND |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene MPND had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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