CRISPR-U™ Gene Knockout Cell Line Strategy

ATG4C Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human ATG4C Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol ATG4C
Gene id 84938
Organism Homo sapiens
Official full symbol autophagy related 4C cysteine peptidase
Gene type protein-coding
Also known as APG4-C, APG4C, AUTL1, AUTL3
Summary Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. This gene encodes a member of the autophagin protein family. The encoded protein is also designated as a member of the C-54 family of cysteine proteases. Alternate transcriptional splice variants, encoding the same protein, have been characterized.
Genomic regions Chromosome 1
Strategy Summary
This gene has 5 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
ATG4C-201 ENST00000317868.9 2944 458aa Protein coding CCDS623 A0A384MTY5 Q96DT6 NM_032852.4 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
ATG4C-203 ENST00000371120.7 2890 458aa Protein coding CCDS623 A0A384MTY5 Q96DT6 - TSL:1, GENCODE basic, APPRIS P1,
ATG4C-202 ENST00000371118.1 665 189aa Protein coding - A6NGQ4 - CDS 3' incomplete, TSL:5,
ATG4C-205 ENST00000443289.5 603 151aa Protein coding - C9JC51 - CDS 3' incomplete, TSL:2,
ATG4C-204 ENST00000414558.2 464 110aa Protein coding - H7BZW5 - CDS 5' incomplete, TSL:5,
Ubigene Red Cotton Transcript
Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene ATG4C
This KO Strategy loading
Red Cotton™ Notes Gene ATG4C had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

Please leave your suggestion ×