CRISPR-U™ Gene Knockout Cell Line
Strategy
ATG4C Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human ATG4C Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | ATG4C |
| Gene id | 84938 |
| Organism | Homo sapiens |
| Official full symbol | autophagy related 4C cysteine peptidase |
| Gene type | protein-coding |
| Also known as | APG4-C, APG4C, AUTL1, AUTL3 |
| Summary | Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. This gene encodes a member of the autophagin protein family. The encoded protein is also designated as a member of the C-54 family of cysteine proteases. Alternate transcriptional splice variants, encoding the same protein, have been characterized. |
| Genomic regions | Chromosome 1 |
Strategy Summary
This gene has 5 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| ATG4C-201 | ENST00000317868.9 | 2944 | 458aa | Protein coding | CCDS623 | A0A384MTY5 Q96DT6 | NM_032852.4 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| ATG4C-203 | ENST00000371120.7 | 2890 | 458aa | Protein coding | CCDS623 | A0A384MTY5 Q96DT6 | - | TSL:1, GENCODE basic, APPRIS P1, |
| ATG4C-202 | ENST00000371118.1 | 665 | 189aa | Protein coding | - | A6NGQ4 | - | CDS 3' incomplete, TSL:5, |
| ATG4C-205 | ENST00000443289.5 | 603 | 151aa | Protein coding | - | C9JC51 | - | CDS 3' incomplete, TSL:2, |
| ATG4C-204 | ENST00000414558.2 | 464 | 110aa | Protein coding | - | H7BZW5 | - | CDS 5' incomplete, TSL:5, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | ATG4C |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene ATG4C had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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