CRISPR-U™ Gene Knockout Cell Line Strategy

PARP10 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human PARP10 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol PARP10
Gene id 84875
Organism Homo sapiens
Official full symbol poly(ADP-ribose) polymerase family member 10
Gene type protein-coding
Also known as ARTD10
Summary Poly(ADP-ribose) polymerases (PARPs), such as PARP10, regulate gene transcription by altering chromatin organization by adding ADP-ribose to histones. PARPs can also function as transcriptional cofactors (Yu et al., 2005 ).
Genomic regions Chromosome 8
Strategy Summary
This gene has 15 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
PARP10-201 ENST00000313028.12 3505 1025aa Protein coding CCDS34960 Q53GL7 NM_032789.5 TSL:1, GENCODE basic, APPRIS P4, MANE Select v0.92,
PARP10-205 ENST00000525773.5 3404 1037aa Protein coding CCDS83331 E9PNI7 - TSL:2, GENCODE basic, APPRIS ALT2,
PARP10-203 ENST00000524918.5 3469 1016aa Protein coding - E9PK67 - TSL:5, GENCODE basic, APPRIS ALT2,
PARP10-202 ENST00000313059.9 1090 339aa Protein coding - F8W8G7 - CDS 3' incomplete, TSL:1,
PARP10-206 ENST00000525879.5 939 178aa Protein coding - E9PPV8 - CDS 3' incomplete, TSL:3,
PARP10-210 ENST00000528136.5 899 128aa Protein coding - E9PM86 - CDS 3' incomplete, TSL:4,
PARP10-216 ENST00000529842.5 696 144aa Protein coding - E9PIA6 - CDS 3' incomplete, TSL:4,
PARP10-212 ENST00000528625.5 685 172aa Protein coding - E9PJI2 - CDS 3' incomplete, TSL:2,
PARP10-213 ENST00000528914.5 589 135aa Protein coding - E9PSE7 - CDS 3' incomplete, TSL:4,
PARP10-217 ENST00000530478.5 588 88aa Protein coding - E9PPU2 - CDS 3' incomplete, TSL:4,
PARP10-218 ENST00000531537.5 578 144aa Protein coding - E9PIA6 - CDS 3' incomplete, TSL:4,
PARP10-204 ENST00000525486.5 564 146aa Protein coding - E9PIK9 - CDS 3' incomplete, TSL:4,
PARP10-215 ENST00000529311.5 529 106aa Protein coding - E9PQQ6 - CDS 3' incomplete, TSL:4,
PARP10-219 ENST00000531707.1 500 81aa Protein coding - E9PSG8 - CDS 3' incomplete, TSL:4,
PARP10-220 ENST00000532311.5 481 83aa Protein coding - E9PLE8 - CDS 3' incomplete, TSL:4,
PARP10-209 ENST00000527262.5 3027 764aa Nonsense mediated decay - E9PPE7 - TSL:1,
PARP10-222 ENST00000533665.1 935 No protein Processed transcript - - - TSL:1,
PARP10-223 ENST00000534737.1 550 No protein Processed transcript - - - TSL:5,
PARP10-221 ENST00000532660.1 545 No protein Processed transcript - - - TSL:4,
PARP10-207 ENST00000526007.5 2179 No protein Retained intron - - - TSL:1,
PARP10-214 ENST00000528963.1 581 No protein Retained intron - - - TSL:2,
PARP10-208 ENST00000526985.1 571 No protein Retained intron - - - TSL:3,
PARP10-211 ENST00000528580.1 488 No protein Retained intron - - - TSL:1,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene PARP10
This KO Strategy loading
Red Cotton™ Notes Gene PARP10 had been KO in a549 cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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