CRISPR-U™ Gene Knockout Cell Line
Strategy
PARP10 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human PARP10 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | PARP10 |
| Gene id | 84875 |
| Organism | Homo sapiens |
| Official full symbol | poly(ADP-ribose) polymerase family member 10 |
| Gene type | protein-coding |
| Also known as | ARTD10 |
| Summary | Poly(ADP-ribose) polymerases (PARPs), such as PARP10, regulate gene transcription by altering chromatin organization by adding ADP-ribose to histones. PARPs can also function as transcriptional cofactors (Yu et al., 2005 ). |
| Genomic regions | Chromosome 8 |
Strategy Summary
This gene has 15 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| PARP10-201 | ENST00000313028.12 | 3505 | 1025aa | Protein coding | CCDS34960 | Q53GL7 | NM_032789.5 | TSL:1, GENCODE basic, APPRIS P4, MANE Select v0.92, |
| PARP10-205 | ENST00000525773.5 | 3404 | 1037aa | Protein coding | CCDS83331 | E9PNI7 | - | TSL:2, GENCODE basic, APPRIS ALT2, |
| PARP10-203 | ENST00000524918.5 | 3469 | 1016aa | Protein coding | - | E9PK67 | - | TSL:5, GENCODE basic, APPRIS ALT2, |
| PARP10-202 | ENST00000313059.9 | 1090 | 339aa | Protein coding | - | F8W8G7 | - | CDS 3' incomplete, TSL:1, |
| PARP10-206 | ENST00000525879.5 | 939 | 178aa | Protein coding | - | E9PPV8 | - | CDS 3' incomplete, TSL:3, |
| PARP10-210 | ENST00000528136.5 | 899 | 128aa | Protein coding | - | E9PM86 | - | CDS 3' incomplete, TSL:4, |
| PARP10-216 | ENST00000529842.5 | 696 | 144aa | Protein coding | - | E9PIA6 | - | CDS 3' incomplete, TSL:4, |
| PARP10-212 | ENST00000528625.5 | 685 | 172aa | Protein coding | - | E9PJI2 | - | CDS 3' incomplete, TSL:2, |
| PARP10-213 | ENST00000528914.5 | 589 | 135aa | Protein coding | - | E9PSE7 | - | CDS 3' incomplete, TSL:4, |
| PARP10-217 | ENST00000530478.5 | 588 | 88aa | Protein coding | - | E9PPU2 | - | CDS 3' incomplete, TSL:4, |
| PARP10-218 | ENST00000531537.5 | 578 | 144aa | Protein coding | - | E9PIA6 | - | CDS 3' incomplete, TSL:4, |
| PARP10-204 | ENST00000525486.5 | 564 | 146aa | Protein coding | - | E9PIK9 | - | CDS 3' incomplete, TSL:4, |
| PARP10-215 | ENST00000529311.5 | 529 | 106aa | Protein coding | - | E9PQQ6 | - | CDS 3' incomplete, TSL:4, |
| PARP10-219 | ENST00000531707.1 | 500 | 81aa | Protein coding | - | E9PSG8 | - | CDS 3' incomplete, TSL:4, |
| PARP10-220 | ENST00000532311.5 | 481 | 83aa | Protein coding | - | E9PLE8 | - | CDS 3' incomplete, TSL:4, |
| PARP10-209 | ENST00000527262.5 | 3027 | 764aa | Nonsense mediated decay | - | E9PPE7 | - | TSL:1, |
| PARP10-222 | ENST00000533665.1 | 935 | No protein | Processed transcript | - | - | - | TSL:1, |
| PARP10-223 | ENST00000534737.1 | 550 | No protein | Processed transcript | - | - | - | TSL:5, |
| PARP10-221 | ENST00000532660.1 | 545 | No protein | Processed transcript | - | - | - | TSL:4, |
| PARP10-207 | ENST00000526007.5 | 2179 | No protein | Retained intron | - | - | - | TSL:1, |
| PARP10-214 | ENST00000528963.1 | 581 | No protein | Retained intron | - | - | - | TSL:2, |
| PARP10-208 | ENST00000526985.1 | 571 | No protein | Retained intron | - | - | - | TSL:3, |
| PARP10-211 | ENST00000528580.1 | 488 | No protein | Retained intron | - | - | - | TSL:1, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | PARP10 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene PARP10 had been KO in a549 cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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