CRISPR-U™ Gene Knockout Cell Line
Strategy
HAVCR2 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human HAVCR2 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | HAVCR2 |
| Gene id | 84868 |
| Organism | Homo sapiens |
| Official full symbol | hepatitis A virus cellular receptor 2 |
| Gene type | protein-coding |
| Also known as | CD366, HAVcr-2, KIM-3, SPTCL, TIM3, TIMD-3, TIMD3, Tim-3 |
| Summary | The protein encoded by this gene belongs to the immunoglobulin superfamily, and TIM family of proteins. CD4-positive T helper lymphocytes can be divided into types 1 (Th1) and 2 (Th2) on the basis of their cytokine secretion patterns. Th1 cells are involved in cell-mediated immunity to intracellular pathogens and delayed-type hypersensitivity reactions, whereas, Th2 cells are involved in the control of extracellular helminthic infections and the promotion of atopic and allergic diseases. This protein is a Th1-specific cell surface protein that regulates macrophage activation, and inhibits Th1-mediated auto- and alloimmune responses, and promotes immunological tolerance. |
| Genomic regions | Chromosome 5 |
Strategy Summary
This gene has 3 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| HAVCR2-201 | ENST00000307851.9 | 2237 | 301aa | Protein coding | CCDS4333 | Q8TDQ0-1 | NM_032782.5 | TSL:1, GENCODE basic, APPRIS P2, MANE Select v0.92, |
| HAVCR2-204 | ENST00000522593.5 | 975 | 273aa | Protein coding | - | E5RHN3 | - | TSL:5, GENCODE basic, APPRIS ALT2, |
| HAVCR2-206 | ENST00000524219.1 | 566 | 74aa | Protein coding | - | E5RFR4 | - | CDS 3' incomplete, TSL:4, |
| HAVCR2-202 | ENST00000517358.1 | 566 | No protein | Processed transcript | - | - | - | TSL:4, |
| HAVCR2-205 | ENST00000522902.1 | 1010 | No protein | Retained intron | - | - | - | TSL:1, |
| HAVCR2-203 | ENST00000521665.1 | 795 | No protein | Retained intron | - | - | - | TSL:1, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | HAVCR2 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene HAVCR2 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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