CRISPR-U™ Gene Knockout Cell Line Strategy

HAVCR2 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human HAVCR2 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol HAVCR2
Gene id 84868
Organism Homo sapiens
Official full symbol hepatitis A virus cellular receptor 2
Gene type protein-coding
Also known as CD366, HAVcr-2, KIM-3, SPTCL, TIM3, TIMD-3, TIMD3, Tim-3
Summary The protein encoded by this gene belongs to the immunoglobulin superfamily, and TIM family of proteins. CD4-positive T helper lymphocytes can be divided into types 1 (Th1) and 2 (Th2) on the basis of their cytokine secretion patterns. Th1 cells are involved in cell-mediated immunity to intracellular pathogens and delayed-type hypersensitivity reactions, whereas, Th2 cells are involved in the control of extracellular helminthic infections and the promotion of atopic and allergic diseases. This protein is a Th1-specific cell surface protein that regulates macrophage activation, and inhibits Th1-mediated auto- and alloimmune responses, and promotes immunological tolerance.
Genomic regions Chromosome 5
Strategy Summary
This gene has 3 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
HAVCR2-201 ENST00000307851.9 2237 301aa Protein coding CCDS4333 Q8TDQ0-1 NM_032782.5 TSL:1, GENCODE basic, APPRIS P2, MANE Select v0.92,
HAVCR2-204 ENST00000522593.5 975 273aa Protein coding - E5RHN3 - TSL:5, GENCODE basic, APPRIS ALT2,
HAVCR2-206 ENST00000524219.1 566 74aa Protein coding - E5RFR4 - CDS 3' incomplete, TSL:4,
HAVCR2-202 ENST00000517358.1 566 No protein Processed transcript - - - TSL:4,
HAVCR2-205 ENST00000522902.1 1010 No protein Retained intron - - - TSL:1,
HAVCR2-203 ENST00000521665.1 795 No protein Retained intron - - - TSL:1,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene HAVCR2
This KO Strategy loading
Red Cotton™ Notes Gene HAVCR2 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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