CRISPR-U™ Gene Knockout Cell Line Strategy

CDC42BPA Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human CDC42BPA Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol CDC42BPA
Gene id 8476
Organism Homo sapiens
Official full symbol CDC42 binding protein kinase alpha
Gene type protein-coding
Also known as MRCK, MRCKA, PK428
Summary The protein encoded by this gene is a member of the serine/threonine protein kinase family. This kinase contains multiple functional domains. Its kinase domain is highly similar to that of the myotonic dystrophy protein kinase (DMPK). This kinase also contains a Rac interactive binding (CRIB) domain, and has been shown to bind CDC42. It may function as a CDC42 downstream effector mediating CDC42 induced peripheral actin formation, and promoting cytoskeletal reorganization. Multiple alternatively spliced transcript variants have been described.
Genomic regions Chromosome 1
Strategy Summary
This gene has 9 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
CDC42BPA-205 ENST00000366769.7 10855 1719aa Protein coding CCDS1558 Q5VT25-5 - TSL:1, GENCODE basic, APPRIS P4,
CDC42BPA-204 ENST00000366767.7 9320 1638aa Protein coding CCDS1559 Q5VT25-3 - TSL:1, GENCODE basic,
CDC42BPA-203 ENST00000366766.6 10610 1018aa Protein coding - A0A0A0MRJ1 - TSL:5, GENCODE basic,
CDC42BPA-202 ENST00000366764.6 10445 1699aa Protein coding - A0A0A0MRJ0 - TSL:1, GENCODE basic,
CDC42BPA-201 ENST00000334218.9 10134 1732aa Protein coding - Q5VT25-1 - TSL:5, GENCODE basic, APPRIS ALT1,
CDC42BPA-209 ENST00000448940.5 7355 984aa Protein coding - H0Y7V8 - CDS 5' incomplete, TSL:1,
CDC42BPA-208 ENST00000442054.5 3786 1048aa Protein coding - H0Y5V1 - CDS 5' incomplete, TSL:1,
CDC42BPA-206 ENST00000429440.5 2501 546aa Protein coding - H0Y6R0 - CDS 5' incomplete, TSL:2,
CDC42BPA-207 ENST00000441725.1 2183 728aa Protein coding - H0Y6V3 - CDS 5' and 3' incomplete, TSL:5,
CDC42BPA-213 ENST00000488131.1 1859 No protein Processed transcript - - - TSL:5,
CDC42BPA-211 ENST00000466538.1 568 No protein Processed transcript - - - TSL:3,
CDC42BPA-210 ENST00000462553.2 430 No protein Processed transcript - - - TSL:5,
CDC42BPA-212 ENST00000478573.6 599 No protein Retained intron - - - TSL:5,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene CDC42BPA
This KO Strategy loading
Red Cotton™ Notes Gene CDC42BPA had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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