CRISPR-U™ Gene Knockout Cell Line
Strategy
CDC42BPA Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human CDC42BPA Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | CDC42BPA |
| Gene id | 8476 |
| Organism | Homo sapiens |
| Official full symbol | CDC42 binding protein kinase alpha |
| Gene type | protein-coding |
| Also known as | MRCK, MRCKA, PK428 |
| Summary | The protein encoded by this gene is a member of the serine/threonine protein kinase family. This kinase contains multiple functional domains. Its kinase domain is highly similar to that of the myotonic dystrophy protein kinase (DMPK). This kinase also contains a Rac interactive binding (CRIB) domain, and has been shown to bind CDC42. It may function as a CDC42 downstream effector mediating CDC42 induced peripheral actin formation, and promoting cytoskeletal reorganization. Multiple alternatively spliced transcript variants have been described. |
| Genomic regions | Chromosome 1 |
Strategy Summary
This gene has 9 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| CDC42BPA-205 | ENST00000366769.7 | 10855 | 1719aa | Protein coding | CCDS1558 | Q5VT25-5 | - | TSL:1, GENCODE basic, APPRIS P4, |
| CDC42BPA-204 | ENST00000366767.7 | 9320 | 1638aa | Protein coding | CCDS1559 | Q5VT25-3 | - | TSL:1, GENCODE basic, |
| CDC42BPA-203 | ENST00000366766.6 | 10610 | 1018aa | Protein coding | - | A0A0A0MRJ1 | - | TSL:5, GENCODE basic, |
| CDC42BPA-202 | ENST00000366764.6 | 10445 | 1699aa | Protein coding | - | A0A0A0MRJ0 | - | TSL:1, GENCODE basic, |
| CDC42BPA-201 | ENST00000334218.9 | 10134 | 1732aa | Protein coding | - | Q5VT25-1 | - | TSL:5, GENCODE basic, APPRIS ALT1, |
| CDC42BPA-209 | ENST00000448940.5 | 7355 | 984aa | Protein coding | - | H0Y7V8 | - | CDS 5' incomplete, TSL:1, |
| CDC42BPA-208 | ENST00000442054.5 | 3786 | 1048aa | Protein coding | - | H0Y5V1 | - | CDS 5' incomplete, TSL:1, |
| CDC42BPA-206 | ENST00000429440.5 | 2501 | 546aa | Protein coding | - | H0Y6R0 | - | CDS 5' incomplete, TSL:2, |
| CDC42BPA-207 | ENST00000441725.1 | 2183 | 728aa | Protein coding | - | H0Y6V3 | - | CDS 5' and 3' incomplete, TSL:5, |
| CDC42BPA-213 | ENST00000488131.1 | 1859 | No protein | Processed transcript | - | - | - | TSL:5, |
| CDC42BPA-211 | ENST00000466538.1 | 568 | No protein | Processed transcript | - | - | - | TSL:3, |
| CDC42BPA-210 | ENST00000462553.2 | 430 | No protein | Processed transcript | - | - | - | TSL:5, |
| CDC42BPA-212 | ENST00000478573.6 | 599 | No protein | Retained intron | - | - | - | TSL:5, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | CDC42BPA |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene CDC42BPA had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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