CRISPR-U™ Gene Knockout Cell Line Strategy

HINT2 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human HINT2 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol HINT2
Gene id 84681
Organism Homo sapiens
Official full symbol histidine triad nucleotide binding protein 2
Gene type protein-coding
Also known as HIT-17
Summary Histidine triad proteins, such as HINT2, are nucleotide hydrolases and transferases that act on the alpha-phosphate of ribonucleotides (Brenner, 2002 ).
Genomic regions Chromosome 9
Strategy Summary
This gene has 1 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
HINT2-201 ENST00000259667.6 649 163aa Protein coding CCDS6594 Q9BX68 NM_032593.3 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
HINT2-206 ENST00000474908.5 2167 No protein Processed transcript - - - TSL:1,
HINT2-205 ENST00000474848.6 853 No protein Processed transcript - - - TSL:2,
HINT2-202 ENST00000461169.1 734 No protein Processed transcript - - - TSL:2,
HINT2-207 ENST00000490578.1 717 No protein Processed transcript - - - TSL:2,
HINT2-204 ENST00000472085.5 569 No protein Processed transcript - - - TSL:3,
HINT2-203 ENST00000471774.5 443 No protein Processed transcript - - - TSL:3,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene HINT2
This KO Strategy loading
Red Cotton™ Notes Gene HINT2 had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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