CRISPR-U™ Gene Knockout Cell Line
Strategy
HINT2 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human HINT2 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | HINT2 |
| Gene id | 84681 |
| Organism | Homo sapiens |
| Official full symbol | histidine triad nucleotide binding protein 2 |
| Gene type | protein-coding |
| Also known as | HIT-17 |
| Summary | Histidine triad proteins, such as HINT2, are nucleotide hydrolases and transferases that act on the alpha-phosphate of ribonucleotides (Brenner, 2002 ). |
| Genomic regions | Chromosome 9 |
Strategy Summary
This gene has 1 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| HINT2-201 | ENST00000259667.6 | 649 | 163aa | Protein coding | CCDS6594 | Q9BX68 | NM_032593.3 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| HINT2-206 | ENST00000474908.5 | 2167 | No protein | Processed transcript | - | - | - | TSL:1, |
| HINT2-205 | ENST00000474848.6 | 853 | No protein | Processed transcript | - | - | - | TSL:2, |
| HINT2-202 | ENST00000461169.1 | 734 | No protein | Processed transcript | - | - | - | TSL:2, |
| HINT2-207 | ENST00000490578.1 | 717 | No protein | Processed transcript | - | - | - | TSL:2, |
| HINT2-204 | ENST00000472085.5 | 569 | No protein | Processed transcript | - | - | - | TSL:3, |
| HINT2-203 | ENST00000471774.5 | 443 | No protein | Processed transcript | - | - | - | TSL:3, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | HINT2 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene HINT2 had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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