CRISPR-U™ Gene Knockout Cell Line Strategy

DGAT2 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human DGAT2 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol DGAT2
Gene id 84649
Organism Homo sapiens
Official full symbol diacylglycerol O-acyltransferase 2
Gene type protein-coding
Also known as ARAT, GS1999FULL, HMFN1045
Summary This gene encodes one of two enzymes which catalyzes the final reaction in the synthesis of triglycerides in which diacylglycerol is covalently bound to long chain fatty acyl-CoAs. The encoded protein catalyzes this reaction at low concentrations of magnesium chloride while the other enzyme has high activity at high concentrations of magnesium chloride. Multiple transcript variants encoding different isoforms have been found for this gene.
Genomic regions Chromosome 11
Strategy Summary
This gene has 6 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
DGAT2-201 ENST00000228027.12 2407 388aa Protein coding CCDS31642 Q96PD7-1 NM_032564.5 TSL:1, GENCODE basic, APPRIS P2, MANE Select v0.92,
DGAT2-202 ENST00000376262.7 2324 345aa Protein coding CCDS58162 Q96PD7-2 - TSL:1, GENCODE basic, APPRIS ALT2,
DGAT2-206 ENST00000604733.5 885 295aa Protein coding - S4R3S3 - CDS 5' and 3' incomplete, TSL:1,
DGAT2-203 ENST00000603276.5 747 160aa Protein coding - S4R383 - CDS 3' incomplete, TSL:5,
DGAT2-207 ENST00000604935.5 618 112aa Protein coding - S4R3Z3 - CDS 3' incomplete, TSL:4,
DGAT2-208 ENST00000605099.1 584 113aa Protein coding - S4R449 - CDS 3' incomplete, TSL:4,
DGAT2-209 ENST00000605608.1 635 No protein Processed transcript - - - TSL:1,
DGAT2-204 ENST00000603363.5 5926 No protein Retained intron - - - TSL:2,
DGAT2-205 ENST00000603865.1 2678 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene DGAT2
This KO Strategy loading
Red Cotton™ Notes Gene DGAT2 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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