CRISPR-U™ Gene Knockout Cell Line
Strategy
DGAT2 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human DGAT2 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | DGAT2 |
| Gene id | 84649 |
| Organism | Homo sapiens |
| Official full symbol | diacylglycerol O-acyltransferase 2 |
| Gene type | protein-coding |
| Also known as | ARAT, GS1999FULL, HMFN1045 |
| Summary | This gene encodes one of two enzymes which catalyzes the final reaction in the synthesis of triglycerides in which diacylglycerol is covalently bound to long chain fatty acyl-CoAs. The encoded protein catalyzes this reaction at low concentrations of magnesium chloride while the other enzyme has high activity at high concentrations of magnesium chloride. Multiple transcript variants encoding different isoforms have been found for this gene. |
| Genomic regions | Chromosome 11 |
Strategy Summary
This gene has 6 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| DGAT2-201 | ENST00000228027.12 | 2407 | 388aa | Protein coding | CCDS31642 | Q96PD7-1 | NM_032564.5 | TSL:1, GENCODE basic, APPRIS P2, MANE Select v0.92, |
| DGAT2-202 | ENST00000376262.7 | 2324 | 345aa | Protein coding | CCDS58162 | Q96PD7-2 | - | TSL:1, GENCODE basic, APPRIS ALT2, |
| DGAT2-206 | ENST00000604733.5 | 885 | 295aa | Protein coding | - | S4R3S3 | - | CDS 5' and 3' incomplete, TSL:1, |
| DGAT2-203 | ENST00000603276.5 | 747 | 160aa | Protein coding | - | S4R383 | - | CDS 3' incomplete, TSL:5, |
| DGAT2-207 | ENST00000604935.5 | 618 | 112aa | Protein coding | - | S4R3Z3 | - | CDS 3' incomplete, TSL:4, |
| DGAT2-208 | ENST00000605099.1 | 584 | 113aa | Protein coding | - | S4R449 | - | CDS 3' incomplete, TSL:4, |
| DGAT2-209 | ENST00000605608.1 | 635 | No protein | Processed transcript | - | - | - | TSL:1, |
| DGAT2-204 | ENST00000603363.5 | 5926 | No protein | Retained intron | - | - | - | TSL:2, |
| DGAT2-205 | ENST00000603865.1 | 2678 | No protein | Retained intron | - | - | - | TSL:2, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | DGAT2 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene DGAT2 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
Comment: