CRISPR-U™ Gene Knockout Cell Line Strategy

L3MBTL3 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human L3MBTL3 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol L3MBTL3
Gene id 84456
Organism Homo sapiens
Official full symbol L3MBTL histone methyl-lysine binding protein 3
Gene type protein-coding
Also known as MBT-1, MBT1
Summary This gene encodes a member of the malignant brain tumor (MBT) family of chromatin interacting transcriptional repressors. Members of this family function as methyl-lysine readers, which recognize methylated lysine residues on histone protein tails, and are associated with the repression of gene expression. The encoded protein may regulate hematopoiesis. Homozygous deletion of this gene has been observed in human patients with medulloblastoma.
Genomic regions Chromosome 6
Strategy Summary
This gene has 8 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
L3MBTL3-202 ENST00000368136.3 4245 780aa Protein coding CCDS34537 Q96JM7-1 - TSL:5, GENCODE basic, APPRIS ALT2,
L3MBTL3-201 ENST00000361794.7 4206 780aa Protein coding CCDS34537 Q96JM7-1 NM_032438.4 TSL:5, GENCODE basic, APPRIS ALT2, MANE Select v0.92,
L3MBTL3-203 ENST00000368139.6 4148 755aa Protein coding CCDS34538 Q96JM7-2 - TSL:5, GENCODE basic, APPRIS P4,
L3MBTL3-204 ENST00000526019.5 3243 755aa Protein coding CCDS34538 Q96JM7-2 - TSL:5, GENCODE basic, APPRIS P4,
L3MBTL3-208 ENST00000533560.5 3165 755aa Protein coding CCDS34538 Q96JM7-2 - TSL:1, GENCODE basic, APPRIS P4,
L3MBTL3-206 ENST00000528385.5 762 190aa Protein coding - E9PLL7 - CDS 3' incomplete, TSL:5,
L3MBTL3-205 ENST00000526087.5 665 163aa Protein coding - E9PI01 - CDS 3' incomplete, TSL:5,
L3MBTL3-210 ENST00000674039.1 537 19aa Protein coding - A0A669KAX0 - CDS 3' incomplete,
L3MBTL3-209 ENST00000533890.1 572 No protein Retained intron - - - TSL:5,
L3MBTL3-207 ENST00000531313.1 544 No protein Retained intron - - - TSL:4,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene L3MBTL3
This KO Strategy loading
Red Cotton™ Notes Gene L3MBTL3 had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
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