CRISPR-U™ Gene Knockout Cell Line
Strategy
L3MBTL3 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human L3MBTL3 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | L3MBTL3 |
| Gene id | 84456 |
| Organism | Homo sapiens |
| Official full symbol | L3MBTL histone methyl-lysine binding protein 3 |
| Gene type | protein-coding |
| Also known as | MBT-1, MBT1 |
| Summary | This gene encodes a member of the malignant brain tumor (MBT) family of chromatin interacting transcriptional repressors. Members of this family function as methyl-lysine readers, which recognize methylated lysine residues on histone protein tails, and are associated with the repression of gene expression. The encoded protein may regulate hematopoiesis. Homozygous deletion of this gene has been observed in human patients with medulloblastoma. |
| Genomic regions | Chromosome 6 |
Strategy Summary
This gene has 8 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| L3MBTL3-202 | ENST00000368136.3 | 4245 | 780aa | Protein coding | CCDS34537 | Q96JM7-1 | - | TSL:5, GENCODE basic, APPRIS ALT2, |
| L3MBTL3-201 | ENST00000361794.7 | 4206 | 780aa | Protein coding | CCDS34537 | Q96JM7-1 | NM_032438.4 | TSL:5, GENCODE basic, APPRIS ALT2, MANE Select v0.92, |
| L3MBTL3-203 | ENST00000368139.6 | 4148 | 755aa | Protein coding | CCDS34538 | Q96JM7-2 | - | TSL:5, GENCODE basic, APPRIS P4, |
| L3MBTL3-204 | ENST00000526019.5 | 3243 | 755aa | Protein coding | CCDS34538 | Q96JM7-2 | - | TSL:5, GENCODE basic, APPRIS P4, |
| L3MBTL3-208 | ENST00000533560.5 | 3165 | 755aa | Protein coding | CCDS34538 | Q96JM7-2 | - | TSL:1, GENCODE basic, APPRIS P4, |
| L3MBTL3-206 | ENST00000528385.5 | 762 | 190aa | Protein coding | - | E9PLL7 | - | CDS 3' incomplete, TSL:5, |
| L3MBTL3-205 | ENST00000526087.5 | 665 | 163aa | Protein coding | - | E9PI01 | - | CDS 3' incomplete, TSL:5, |
| L3MBTL3-210 | ENST00000674039.1 | 537 | 19aa | Protein coding | - | A0A669KAX0 | - | CDS 3' incomplete, |
| L3MBTL3-209 | ENST00000533890.1 | 572 | No protein | Retained intron | - | - | - | TSL:5, |
| L3MBTL3-207 | ENST00000531313.1 | 544 | No protein | Retained intron | - | - | - | TSL:4, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | L3MBTL3 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene L3MBTL3 had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
Comment: