CRISPR-U™ Gene Knockout Cell Line Strategy

BRSK1 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human BRSK1 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol BRSK1
Gene id 84446
Organism Homo sapiens
Official full symbol BR serine/threonine kinase 1
Gene type protein-coding
Also known as hSAD1
Genomic regions Chromosome 19
Strategy Summary
This gene has 5 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
BRSK1-201 ENST00000309383.6 3277 778aa Protein coding CCDS12921 Q8TDC3-1 NM_032430.2 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
BRSK1-206 ENST00000590333.5 2977 794aa Protein coding - Q8TDC3-2 - TSL:1, GENCODE basic,
BRSK1-202 ENST00000326848.7 2095 473aa Protein coding - J3KNK0 - TSL:5, GENCODE basic,
BRSK1-203 ENST00000585418.1 1104 343aa Protein coding - Q8TDC3-3 - TSL:1, GENCODE basic,
BRSK1-207 ENST00000591774.5 556 131aa Protein coding - K7EM68 - CDS 3' incomplete, TSL:4,
BRSK1-208 ENST00000592539.6 545 63aa Nonsense mediated decay - K7EN26 - CDS 5' incomplete, TSL:5,
BRSK1-205 ENST00000588584.1 547 No protein Processed transcript - - - TSL:5,
BRSK1-204 ENST00000586626.1 540 No protein Retained intron - - - TSL:3,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene BRSK1
This KO Strategy loading
Red Cotton™ Notes Gene BRSK1 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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