CRISPR-U™ Gene Knockout Cell Line
Strategy
BRSK1 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human BRSK1 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | BRSK1 |
| Gene id | 84446 |
| Organism | Homo sapiens |
| Official full symbol | BR serine/threonine kinase 1 |
| Gene type | protein-coding |
| Also known as | hSAD1 |
| Genomic regions | Chromosome 19 |
Strategy Summary
This gene has 5 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| BRSK1-201 | ENST00000309383.6 | 3277 | 778aa | Protein coding | CCDS12921 | Q8TDC3-1 | NM_032430.2 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| BRSK1-206 | ENST00000590333.5 | 2977 | 794aa | Protein coding | - | Q8TDC3-2 | - | TSL:1, GENCODE basic, |
| BRSK1-202 | ENST00000326848.7 | 2095 | 473aa | Protein coding | - | J3KNK0 | - | TSL:5, GENCODE basic, |
| BRSK1-203 | ENST00000585418.1 | 1104 | 343aa | Protein coding | - | Q8TDC3-3 | - | TSL:1, GENCODE basic, |
| BRSK1-207 | ENST00000591774.5 | 556 | 131aa | Protein coding | - | K7EM68 | - | CDS 3' incomplete, TSL:4, |
| BRSK1-208 | ENST00000592539.6 | 545 | 63aa | Nonsense mediated decay | - | K7EN26 | - | CDS 5' incomplete, TSL:5, |
| BRSK1-205 | ENST00000588584.1 | 547 | No protein | Processed transcript | - | - | - | TSL:5, |
| BRSK1-204 | ENST00000586626.1 | 540 | No protein | Retained intron | - | - | - | TSL:3, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | BRSK1 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene BRSK1 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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