CRISPR-U™ Gene Knockout Cell Line Strategy

GNPAT Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human GNPAT Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol GNPAT
Gene id 8443
Organism Homo sapiens
Official full symbol glyceronephosphate O-acyltransferase
Gene type protein-coding
Also known as DAP-AT, DAPAT, DHAPAT, RCDP2
Summary This gene encodes an enzyme located in the peroxisomal membrane which is essential to the synthesis of ether phospholipids. Mutations in this gene are associated with rhizomelic chondrodysplasia punctata. Two transcript variants encoding different isoforms have been found for this gene.
Genomic regions Chromosome 1
Strategy Summary
This gene has 3 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
GNPAT-201 ENST00000366647.9 2641 680aa Protein coding CCDS1592 O15228-1 NM_014236.4 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
GNPAT-202 ENST00000416000.1 1924 587aa Protein coding - Q5TBH8 - CDS 3' incomplete, TSL:5,
GNPAT-203 ENST00000436239.5 839 222aa Protein coding - Q5TBH6 - CDS 3' incomplete, TSL:3,
GNPAT-206 ENST00000644483.1 2685 133aa Nonsense mediated decay - A0A2R8YH69 - -
GNPAT-204 ENST00000469332.1 613 No protein Processed transcript - - - TSL:2,
GNPAT-205 ENST00000492459.1 589 No protein Processed transcript - - - TSL:2,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene GNPAT
This KO Strategy loading
Red Cotton™ Notes Gene GNPAT had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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