CRISPR-U™ Gene Knockout Cell Line
Strategy
CYSTM1 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human CYSTM1 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | CYSTM1 |
| Gene id | 84418 |
| Organism | Homo sapiens |
| Official full symbol | cysteine rich transmembrane module containing 1 |
| Gene type | protein-coding |
| Also known as | C5orf32, ORF1-FL49 |
| Genomic regions | Chromosome 5 |
Strategy Summary
This gene has 2 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| CYSTM1-201 | ENST00000261811.6 | 790 | 97aa | Protein coding | CCDS4221 | Q9H1C7 | NM_032412.4 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| CYSTM1-205 | ENST00000644078.1 | 462 | 97aa | Protein coding | - | A0A2R8YEW2 | - | GENCODE basic, |
| CYSTM1-204 | ENST00000509789.2 | 813 | No protein | Processed transcript | - | - | - | TSL:4, |
| CYSTM1-203 | ENST00000509589.5 | 665 | No protein | Processed transcript | - | - | - | TSL:3, |
| CYSTM1-202 | ENST00000504227.5 | 588 | No protein | Processed transcript | - | - | - | TSL:5, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | CYSTM1 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene CYSTM1 had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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