CRISPR-U™ Gene Knockout Cell Line Strategy

CYSTM1 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human CYSTM1 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol CYSTM1
Gene id 84418
Organism Homo sapiens
Official full symbol cysteine rich transmembrane module containing 1
Gene type protein-coding
Also known as C5orf32, ORF1-FL49
Genomic regions Chromosome 5
Strategy Summary
This gene has 2 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
CYSTM1-201 ENST00000261811.6 790 97aa Protein coding CCDS4221 Q9H1C7 NM_032412.4 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
CYSTM1-205 ENST00000644078.1 462 97aa Protein coding - A0A2R8YEW2 - GENCODE basic,
CYSTM1-204 ENST00000509789.2 813 No protein Processed transcript - - - TSL:4,
CYSTM1-203 ENST00000509589.5 665 No protein Processed transcript - - - TSL:3,
CYSTM1-202 ENST00000504227.5 588 No protein Processed transcript - - - TSL:5,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene CYSTM1
This KO Strategy loading
Red Cotton™ Notes Gene CYSTM1 had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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