CRISPR-U™ Gene Knockout Cell Line Strategy

ELOF1 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human ELOF1 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol ELOF1
Gene id 84337
Organism Homo sapiens
Official full symbol elongation factor 1 homolog
Gene type protein-coding
Also known as ELF1
Genomic regions Chromosome 19
Strategy Summary
This gene has 9 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
ELOF1-204 ENST00000587806.5 1172 104aa Protein coding CCDS86703 A0A024R7E8 - TSL:2, GENCODE basic,
ELOF1-201 ENST00000252445.7 1019 83aa Protein coding CCDS12264 P60002 - TSL:2, GENCODE basic, APPRIS P1,
ELOF1-202 ENST00000586120.5 658 83aa Protein coding CCDS12264 P60002 - TSL:3, GENCODE basic, APPRIS P1,
ELOF1-206 ENST00000590700.5 585 83aa Protein coding CCDS12264 P60002 - TSL:3, GENCODE basic, APPRIS P1,
ELOF1-203 ENST00000586683.5 2154 114aa Protein coding - K7EQ44 - TSL:2, GENCODE basic,
ELOF1-208 ENST00000591912.1 899 120aa Protein coding - K7EMV4 - TSL:5, GENCODE basic,
ELOF1-205 ENST00000589171.5 743 76aa Protein coding - K7EN05 - TSL:3, GENCODE basic,
ELOF1-207 ENST00000591674.5 679 90aa Protein coding - K7EPC8 - TSL:2, GENCODE basic,
ELOF1-209 ENST00000593077.1 585 14aa Protein coding - K7EL94 - CDS 3' incomplete, TSL:4,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene ELOF1
This KO Strategy loading
Red Cotton™ Notes Gene ELOF1 had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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