CRISPR-U™ Gene Knockout Cell Line Strategy

CHCHD6 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human CHCHD6 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol CHCHD6
Gene id 84303
Organism Homo sapiens
Official full symbol coiled-coil-helix-coiled-coil-helix domain containing 6
Gene type protein-coding
Also known as CHCM1, MICOS25, Mic25, PPP1R23
Genomic regions Chromosome 3
Strategy Summary
This gene has 3 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
CHCHD6-201 ENST00000290913.8 1000 235aa Protein coding CCDS3041 Q9BRQ6 NM_032343.3 TSL:1, GENCODE basic, APPRIS P4, MANE Select v0.92,
CHCHD6-204 ENST00000508789.5 1159 256aa Protein coding - J3QTA6 - TSL:1, GENCODE basic, APPRIS ALT2,
CHCHD6-206 ENST00000513253.1 690 166aa Protein coding - H0Y922 - CDS 5' incomplete, TSL:3,
CHCHD6-202 ENST00000503119.5 1148 140aa Nonsense mediated decay - J3QTB2 - TSL:1,
CHCHD6-208 ENST00000515867.1 778 No protein Processed transcript - - - TSL:5,
CHCHD6-207 ENST00000514908.5 713 No protein Processed transcript - - - TSL:3,
CHCHD6-205 ENST00000510044.1 670 No protein Processed transcript - - - TSL:2,
CHCHD6-203 ENST00000504973.5 562 No protein Processed transcript - - - TSL:3,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene CHCHD6
This KO Strategy loading
Red Cotton™ Notes Gene CHCHD6 had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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