CRISPR-U™ Gene Knockout Cell Line
Strategy
NTPCR Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human NTPCR Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | NTPCR |
| Gene id | 84284 |
| Organism | Homo sapiens |
| Official full symbol | nucleoside-triphosphatase, cancer-related |
| Gene type | protein-coding |
| Also known as | C1orf57, HCR-NTPase, THEP1 |
| Summary | The protein encoded by this gene is a non-specific nucleoside triphosphatase that is slow-acting in vitro. This gene is overexpressed in many tumor tissues, and while it is not essential for the cell, overexpression is cytotoxic. However, the cytotoxicity is not related to its triphosphatase activity. |
| Genomic regions | Chromosome 1 |
Strategy Summary
This gene has 2 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| NTPCR-202 | ENST00000366628.10 | 6324 | 190aa | Protein coding | CCDS1597 | Q5TDE9 Q9BSD7 | NM_032324.3 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| NTPCR-201 | ENST00000366627.4 | 1762 | 228aa | Protein coding | - | Q5TDF0 | - | TSL:3, GENCODE basic, |
| NTPCR-205 | ENST00000490098.1 | 8705 | No protein | Processed transcript | - | - | - | TSL:1, |
| NTPCR-206 | ENST00000490807.5 | 980 | No protein | Processed transcript | - | - | - | TSL:2, |
| NTPCR-208 | ENST00000496662.5 | 882 | No protein | Processed transcript | - | - | - | TSL:2, |
| NTPCR-207 | ENST00000494689.5 | 767 | No protein | Processed transcript | - | - | - | TSL:2, |
| NTPCR-203 | ENST00000479125.5 | 761 | No protein | Processed transcript | - | - | - | TSL:3, |
| NTPCR-204 | ENST00000487953.1 | 527 | No protein | Processed transcript | - | - | - | TSL:3, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | NTPCR |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene NTPCR had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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