CRISPR-U™ Gene Knockout Cell Line
Strategy
POLDIP3 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human POLDIP3 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | POLDIP3 |
| Gene id | 84271 |
| Organism | Homo sapiens |
| Official full symbol | DNA polymerase delta interacting protein 3 |
| Gene type | protein-coding |
| Also known as | PDIP3, PDIP46, SKAR |
| Summary | This gene encodes an RRM (RNA recognition motif)-containing protein that participates in the regulation of translation by recruiting ribosomal protein S6 kinase beta-1 to mRNAs. Alternative splicing results in multiple transcript variants. |
| Genomic regions | Chromosome 22 |
Strategy Summary
This gene has 5 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| POLDIP3-205 | ENST00000451060.6 | 3491 | 438aa | Protein coding | CCDS74873 | F6VRR5 | - | TSL:2, GENCODE basic, |
| POLDIP3-201 | ENST00000252115.10 | 3362 | 421aa | Protein coding | CCDS14038 | Q9BY77-1 | NM_032311.5 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| POLDIP3-203 | ENST00000348657.6 | 3323 | 392aa | Protein coding | CCDS14039 | Q9BY77-2 | - | TSL:1, GENCODE basic, |
| POLDIP3-209 | ENST00000617178.4 | 2006 | 229aa | Protein coding | - | Q8WUT1 | - | TSL:1, GENCODE basic, |
| POLDIP3-202 | ENST00000339677.10 | 783 | 185aa | Protein coding | - | Q6R954 | - | TSL:1, GENCODE basic, |
| POLDIP3-204 | ENST00000445215.5 | 3337 | 281aa | Nonsense mediated decay | - | F8WCX5 | - | TSL:1, |
| POLDIP3-206 | ENST00000454057.1 | 579 | 189aa | Nonsense mediated decay | - | F8WEE2 | - | TSL:4, |
| POLDIP3-208 | ENST00000491021.1 | 1028 | No protein | Processed transcript | - | - | - | TSL:5, |
| POLDIP3-207 | ENST00000463133.1 | 563 | No protein | Processed transcript | - | - | - | TSL:4, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | POLDIP3 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene POLDIP3 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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