CRISPR-U™ Gene Knockout Cell Line Strategy

POLDIP3 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human POLDIP3 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol POLDIP3
Gene id 84271
Organism Homo sapiens
Official full symbol DNA polymerase delta interacting protein 3
Gene type protein-coding
Also known as PDIP3, PDIP46, SKAR
Summary This gene encodes an RRM (RNA recognition motif)-containing protein that participates in the regulation of translation by recruiting ribosomal protein S6 kinase beta-1 to mRNAs. Alternative splicing results in multiple transcript variants.
Genomic regions Chromosome 22
Strategy Summary
This gene has 5 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
POLDIP3-205 ENST00000451060.6 3491 438aa Protein coding CCDS74873 F6VRR5 - TSL:2, GENCODE basic,
POLDIP3-201 ENST00000252115.10 3362 421aa Protein coding CCDS14038 Q9BY77-1 NM_032311.5 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
POLDIP3-203 ENST00000348657.6 3323 392aa Protein coding CCDS14039 Q9BY77-2 - TSL:1, GENCODE basic,
POLDIP3-209 ENST00000617178.4 2006 229aa Protein coding - Q8WUT1 - TSL:1, GENCODE basic,
POLDIP3-202 ENST00000339677.10 783 185aa Protein coding - Q6R954 - TSL:1, GENCODE basic,
POLDIP3-204 ENST00000445215.5 3337 281aa Nonsense mediated decay - F8WCX5 - TSL:1,
POLDIP3-206 ENST00000454057.1 579 189aa Nonsense mediated decay - F8WEE2 - TSL:4,
POLDIP3-208 ENST00000491021.1 1028 No protein Processed transcript - - - TSL:5,
POLDIP3-207 ENST00000463133.1 563 No protein Processed transcript - - - TSL:4,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene POLDIP3
This KO Strategy loading
Red Cotton™ Notes Gene POLDIP3 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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