CRISPR-U™ Gene Knockout Cell Line Strategy

MAF1 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human MAF1 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol MAF1
Gene id 84232
Organism Homo sapiens
Official full symbol MAF1 homolog, negative regulator of RNA polymerase III
Gene type protein-coding
Summary This gene encodes a protein that is similar to Maf1, a Saccharomyces cerevisiae protein highly conserved in eukaryotic cells. Yeast Maf1 is a negative effector of RNA polymerase III (Pol III). It responds to changes in the cellular environment and represses pol III transcription. Biochemical studies identified the initiation factor TFIIIB as a target for Maf1-dependent repression.
Genomic regions Chromosome 8
Strategy Summary
This gene has 6 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
MAF1-201 ENST00000322428.10 1715 256aa Protein coding CCDS6416 Q9H063 NM_032272.5 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
MAF1-204 ENST00000532522.5 1098 256aa Protein coding CCDS6416 Q9H063 - TSL:5, GENCODE basic, APPRIS P1,
MAF1-205 ENST00000534585.5 1754 286aa Protein coding - E9PSH4 - TSL:5, GENCODE basic,
MAF1-202 ENST00000527058.1 868 252aa Protein coding - E9PR76 - CDS 3' incomplete, TSL:3,
MAF1-203 ENST00000527572.5 737 70aa Protein coding - E9PJ05 - CDS 3' incomplete, TSL:3,
MAF1-206 ENST00000534811.1 632 164aa Protein coding - H0YEV4 - CDS 5' incomplete, TSL:2,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene MAF1
This KO Strategy loading
Red Cotton™ Notes Gene MAF1 had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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