CRISPR-U™ Gene Knockout Cell Line
Strategy
MAF1 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human MAF1 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | MAF1 |
| Gene id | 84232 |
| Organism | Homo sapiens |
| Official full symbol | MAF1 homolog, negative regulator of RNA polymerase III |
| Gene type | protein-coding |
| Summary | This gene encodes a protein that is similar to Maf1, a Saccharomyces cerevisiae protein highly conserved in eukaryotic cells. Yeast Maf1 is a negative effector of RNA polymerase III (Pol III). It responds to changes in the cellular environment and represses pol III transcription. Biochemical studies identified the initiation factor TFIIIB as a target for Maf1-dependent repression. |
| Genomic regions | Chromosome 8 |
Strategy Summary
This gene has 6 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| MAF1-201 | ENST00000322428.10 | 1715 | 256aa | Protein coding | CCDS6416 | Q9H063 | NM_032272.5 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| MAF1-204 | ENST00000532522.5 | 1098 | 256aa | Protein coding | CCDS6416 | Q9H063 | - | TSL:5, GENCODE basic, APPRIS P1, |
| MAF1-205 | ENST00000534585.5 | 1754 | 286aa | Protein coding | - | E9PSH4 | - | TSL:5, GENCODE basic, |
| MAF1-202 | ENST00000527058.1 | 868 | 252aa | Protein coding | - | E9PR76 | - | CDS 3' incomplete, TSL:3, |
| MAF1-203 | ENST00000527572.5 | 737 | 70aa | Protein coding | - | E9PJ05 | - | CDS 3' incomplete, TSL:3, |
| MAF1-206 | ENST00000534811.1 | 632 | 164aa | Protein coding | - | H0YEV4 | - | CDS 5' incomplete, TSL:2, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | MAF1 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene MAF1 had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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