CRISPR-U™ Gene Knockout Cell Line
Strategy
MEX3B Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human MEX3B Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | MEX3B |
| Gene id | 84206 |
| Organism | Homo sapiens |
| Official full symbol | mex-3 RNA binding family member B |
| Gene type | protein-coding |
| Also known as | MEX-3B, RKHD3, RNF195 |
| Summary | This gene encodes an RNA-binding phosphoprotein that is part of the MEX3 (muscle excess 3) family of translational regulators. The encoded protein contains N-terminal nuclear export and nuclear localization signals and is exported from the cytoplasm to the nucleus. The protein binds to RNA via two KH domains and also colocalizes with MEX3A, Dcp1A decapping factor and Argonaute proteins within P (processing) bodies. |
| Genomic regions | Chromosome 15 |
Strategy Summary
This gene has 2 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| MEX3B-201 | ENST00000329713.5 | 3405 | 569aa | Protein coding | CCDS10319 | Q6ZN04-1 | NM_032246.6 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| MEX3B-202 | ENST00000558133.1 | 4333 | 158aa | Protein coding | - | Q6ZN04-3 | - | TSL:NA, GENCODE basic, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | MEX3B |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene MEX3B had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
Comment: