CRISPR-U™ Gene Knockout Cell Line
Strategy
POMK Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human POMK Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | POMK |
| Gene id | 84197 |
| Organism | Homo sapiens |
| Official full symbol | protein O-mannose kinase |
| Gene type | protein-coding |
| Also known as | MDDGA12, MDDGC12, SGK196 |
| Summary | This gene encodes a protein that may be involved in the presentation of the laminin-binding O-linked carbohydrate chain of alpha-dystroglycan (a-DG), which forms transmembrane linkages between the extracellular matrix and the exoskeleton. Some pathogens use this O-linked carbohydrate unit for host entry. Loss of function compound heterozygous mutations in this gene were found in a human patient affected by the Walker-Warburg syndrome (WWS) phenotype. Mice lacking this gene contain misplaced neurons (heterotopia) in some regions of the brain, possibly from defects in neuronal migration. Alternative splicing of this gene results in multiple transcript variants. |
| Genomic regions | Chromosome 8 |
Strategy Summary
This gene has 8 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| POMK-219 | ENST00000676193.1 | 9535 | 350aa | Protein coding | CCDS6141 | - | - | GENCODE basic, APPRIS P1, |
| POMK-201 | ENST00000331373.10 | 1868 | 350aa | Protein coding | CCDS6141 | Q9H5K3 | NM_032237.5 | TSL:2, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| POMK-209 | ENST00000674937.1 | 3922 | 336aa | Protein coding | - | - | - | GENCODE basic, |
| POMK-215 | ENST00000675675.1 | 2405 | 256aa | Protein coding | - | - | - | CDS 5' incomplete, |
| POMK-205 | ENST00000674676.1 | 1951 | 256aa | Protein coding | - | - | - | CDS 5' incomplete, |
| POMK-211 | ENST00000675129.1 | 625 | 94aa | Protein coding | - | - | - | CDS 3' incomplete, |
| POMK-208 | ENST00000674820.1 | 353 | 94aa | Protein coding | - | - | - | CDS 3' incomplete, |
| POMK-202 | ENST00000518991.6 | 352 | 94aa | Protein coding | - | E5RJD5 | - | CDS 3' incomplete, TSL:4, |
| POMK-212 | ENST00000675322.1 | 3595 | 256aa | Nonsense mediated decay | - | - | - | CDS 5' incomplete, |
| POMK-204 | ENST00000674646.1 | 3576 | 256aa | Nonsense mediated decay | - | - | - | CDS 5' incomplete, |
| POMK-207 | ENST00000674782.1 | 1917 | 121aa | Nonsense mediated decay | - | - | - | - |
| POMK-218 | ENST00000676178.1 | 1899 | 160aa | Nonsense mediated decay | - | - | - | - |
| POMK-203 | ENST00000614426.2 | 1712 | 94aa | Nonsense mediated decay | - | Q9H5K3 | - | TSL:4, |
| POMK-216 | ENST00000675791.1 | 273 | No protein | Processed transcript | - | - | - | - |
| POMK-214 | ENST00000675509.1 | 146 | No protein | Processed transcript | - | - | - | - |
| POMK-217 | ENST00000676163.1 | 145 | No protein | Processed transcript | - | - | - | - |
| POMK-210 | ENST00000675013.1 | 126 | No protein | Processed transcript | - | - | - | - |
| POMK-220 | ENST00000676356.1 | 122 | No protein | Processed transcript | - | - | - | - |
| POMK-213 | ENST00000675501.1 | 120 | No protein | Processed transcript | - | - | - | - |
| POMK-206 | ENST00000674727.1 | 2342 | No protein | Retained intron | - | - | - | - |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | POMK |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene POMK had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
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$599
Single gRNA lentivirus
Work flow
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