CRISPR-U™ Gene Knockout Cell Line Strategy

POMK Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human POMK Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol POMK
Gene id 84197
Organism Homo sapiens
Official full symbol protein O-mannose kinase
Gene type protein-coding
Also known as MDDGA12, MDDGC12, SGK196
Summary This gene encodes a protein that may be involved in the presentation of the laminin-binding O-linked carbohydrate chain of alpha-dystroglycan (a-DG), which forms transmembrane linkages between the extracellular matrix and the exoskeleton. Some pathogens use this O-linked carbohydrate unit for host entry. Loss of function compound heterozygous mutations in this gene were found in a human patient affected by the Walker-Warburg syndrome (WWS) phenotype. Mice lacking this gene contain misplaced neurons (heterotopia) in some regions of the brain, possibly from defects in neuronal migration. Alternative splicing of this gene results in multiple transcript variants.
Genomic regions Chromosome 8
Strategy Summary
This gene has 8 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
POMK-219 ENST00000676193.1 9535 350aa Protein coding CCDS6141 - - GENCODE basic, APPRIS P1,
POMK-201 ENST00000331373.10 1868 350aa Protein coding CCDS6141 Q9H5K3 NM_032237.5 TSL:2, GENCODE basic, APPRIS P1, MANE Select v0.92,
POMK-209 ENST00000674937.1 3922 336aa Protein coding - - - GENCODE basic,
POMK-215 ENST00000675675.1 2405 256aa Protein coding - - - CDS 5' incomplete,
POMK-205 ENST00000674676.1 1951 256aa Protein coding - - - CDS 5' incomplete,
POMK-211 ENST00000675129.1 625 94aa Protein coding - - - CDS 3' incomplete,
POMK-208 ENST00000674820.1 353 94aa Protein coding - - - CDS 3' incomplete,
POMK-202 ENST00000518991.6 352 94aa Protein coding - E5RJD5 - CDS 3' incomplete, TSL:4,
POMK-212 ENST00000675322.1 3595 256aa Nonsense mediated decay - - - CDS 5' incomplete,
POMK-204 ENST00000674646.1 3576 256aa Nonsense mediated decay - - - CDS 5' incomplete,
POMK-207 ENST00000674782.1 1917 121aa Nonsense mediated decay - - - -
POMK-218 ENST00000676178.1 1899 160aa Nonsense mediated decay - - - -
POMK-203 ENST00000614426.2 1712 94aa Nonsense mediated decay - Q9H5K3 - TSL:4,
POMK-216 ENST00000675791.1 273 No protein Processed transcript - - - -
POMK-214 ENST00000675509.1 146 No protein Processed transcript - - - -
POMK-217 ENST00000676163.1 145 No protein Processed transcript - - - -
POMK-210 ENST00000675013.1 126 No protein Processed transcript - - - -
POMK-220 ENST00000676356.1 122 No protein Processed transcript - - - -
POMK-213 ENST00000675501.1 120 No protein Processed transcript - - - -
POMK-206 ENST00000674727.1 2342 No protein Retained intron - - - -
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene POMK
This KO Strategy loading
Red Cotton™ Notes Gene POMK had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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