CRISPR-U™ Gene Knockout Cell Line Strategy

ANTXR1 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human ANTXR1 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol ANTXR1
Gene id 84168
Organism Homo sapiens
Official full symbol ANTXR cell adhesion molecule 1
Gene type protein-coding
Also known as ATR, GAPO, TEM8
Summary This gene encodes a type I transmembrane protein and is a tumor-specific endothelial marker that has been implicated in colorectal cancer. The encoded protein has been shown to also be a docking protein or receptor for Bacillus anthracis toxin, the causative agent of the disease, anthrax. The binding of the protective antigen (PA) component, of the tripartite anthrax toxin, to this receptor protein mediates delivery of toxin components to the cytosol of cells. Once inside the cell, the other two components of anthrax toxin, edema factor (EF) and lethal factor (LF) disrupt normal cellular processes. Three alternatively spliced variants that encode different protein isoforms have been described.
Genomic regions Chromosome 2
Strategy Summary
This gene has 7 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
ANTXR1-201 ENST00000303714.9 5858 564aa Protein coding CCDS1892 Q9H6X2-1 NM_032208.3 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
ANTXR1-203 ENST00000409829.7 2221 333aa Protein coding CCDS46314 Q9H6X2-4 - TSL:1, GENCODE basic,
ANTXR1-202 ENST00000409349.7 1384 368aa Protein coding CCDS46313 Q9H6X2-2 - TSL:1, GENCODE basic,
ANTXR1-211 ENST00000681816.1 1989 346aa Protein coding - - - GENCODE basic,
ANTXR1-204 ENST00000463335.2 1449 297aa Protein coding - - - TSL:1, GENCODE basic,
ANTXR1-208 ENST00000679548.1 1261 351aa Protein coding - - - CDS 5' incomplete,
ANTXR1-206 ENST00000482235.2 1126 347aa Protein coding - H0YC24 - TSL:3, GENCODE basic,
ANTXR1-209 ENST00000681059.1 3862 No protein Processed transcript - - - -
ANTXR1-210 ENST00000681568.1 4853 No protein Retained intron - - - -
ANTXR1-205 ENST00000481119.2 2955 No protein Retained intron - - - TSL:3,
ANTXR1-207 ENST00000497197.3 480 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene ANTXR1
This KO Strategy loading
Red Cotton™ Notes Gene ANTXR1 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
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Work flow
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