CRISPR-U™ Gene Knockout Cell Line
Strategy
NLRC5 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human NLRC5 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | NLRC5 |
| Gene id | 84166 |
| Organism | Homo sapiens |
| Official full symbol | NLR family CARD domain containing 5 |
| Gene type | protein-coding |
| Also known as | CLR16.1, NOD27, NOD4 |
| Summary | This gene encodes a member of the caspase recruitment domain-containing NLR family. This gene plays a role in cytokine response and antiviral immunity through its inhibition of NF-kappa-B activation and negative regulation of type I interferon signaling pathways. |
| Genomic regions | Chromosome 16 |
Strategy Summary
This gene has 8 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| NLRC5-201 | ENST00000262510.10 | 6822 | 1866aa | Protein coding | CCDS10773 | Q86WI3-1 | - | TSL:5, GENCODE basic, APPRIS P4, |
| NLRC5-216 | ENST00000539144.5 | 6505 | 1837aa | Protein coding | CCDS81985 | Q86WI3-4 | - | TSL:5, GENCODE basic, APPRIS ALT2, |
| NLRC5-214 | ENST00000538805.5 | 3552 | 1180aa | Protein coding | - | H0YGE2 | - | CDS 5' incomplete, TSL:2, |
| NLRC5-210 | ENST00000538110.5 | 2656 | 885aa | Protein coding | - | H0YG32 | - | CDS 5' and 3' incomplete, TSL:5, |
| NLRC5-220 | ENST00000543030.5 | 1894 | 631aa | Protein coding | - | H0YFT1 | - | CDS 5' and 3' incomplete, TSL:5, |
| NLRC5-226 | ENST00000544641.5 | 594 | 74aa | Protein coding | - | F5H274 | - | CDS 3' incomplete, TSL:3, |
| NLRC5-202 | ENST00000399221.3 | 550 | 157aa | Protein coding | - | H0YFT3 | - | CDS 5' incomplete, TSL:3, |
| NLRC5-209 | ENST00000538059.5 | 282 | 19aa | Protein coding | - | F5GYP0 | - | CDS 3' incomplete, TSL:3, |
| NLRC5-227 | ENST00000545081.5 | 5832 | 1057aa | Nonsense mediated decay | - | H0YGM1 | - | CDS 5' incomplete, TSL:2, |
| NLRC5-217 | ENST00000539881.5 | 4777 | 720aa | Nonsense mediated decay | - | Q86WI3-3 | - | TSL:2, |
| NLRC5-212 | ENST00000538453.5 | 4121 | 702aa | Nonsense mediated decay | - | H0YGT6 | - | CDS 5' incomplete, TSL:2, |
| NLRC5-218 | ENST00000540182.5 | 3617 | 387aa | Nonsense mediated decay | - | H0YFG4 | - | CDS 5' incomplete, TSL:1, |
| NLRC5-208 | ENST00000537056.5 | 3430 | 325aa | Nonsense mediated decay | - | H0YF88 | - | CDS 5' incomplete, TSL:1, |
| NLRC5-228 | ENST00000545349.1 | 485 | 110aa | Nonsense mediated decay | - | H0YGV6 | - | CDS 5' incomplete, TSL:5, |
| NLRC5-223 | ENST00000543141.5 | 563 | No protein | Processed transcript | - | - | - | TSL:4, |
| NLRC5-206 | ENST00000535658.1 | 527 | No protein | Processed transcript | - | - | - | TSL:5, |
| NLRC5-222 | ENST00000543103.1 | 1896 | No protein | Retained intron | - | - | - | TSL:2, |
| NLRC5-215 | ENST00000538930.5 | 1686 | No protein | Retained intron | - | - | - | TSL:5, |
| NLRC5-203 | ENST00000534927.5 | 1051 | No protein | Retained intron | - | - | - | TSL:5, |
| NLRC5-213 | ENST00000538778.5 | 1019 | No protein | Retained intron | - | - | - | TSL:3, |
| NLRC5-205 | ENST00000535284.1 | 907 | No protein | Retained intron | - | - | - | TSL:3, |
| NLRC5-207 | ENST00000536231.1 | 868 | No protein | Retained intron | - | - | - | TSL:5, |
| NLRC5-224 | ENST00000543402.1 | 575 | No protein | Retained intron | - | - | - | TSL:2, |
| NLRC5-211 | ENST00000538273.5 | 560 | No protein | Retained intron | - | - | - | TSL:2, |
| NLRC5-204 | ENST00000534931.1 | 549 | No protein | Retained intron | - | - | - | TSL:4, |
| NLRC5-225 | ENST00000544189.1 | 546 | No protein | Retained intron | - | - | - | TSL:4, |
| NLRC5-221 | ENST00000543049.1 | 484 | No protein | Retained intron | - | - | - | TSL:2, |
| NLRC5-219 | ENST00000541020.1 | 465 | No protein | Retained intron | - | - | - | TSL:3, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | NLRC5 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene NLRC5 had been KO in a549 cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
Comment: