CRISPR-U™ Gene Knockout Cell Line Strategy

BCAR3 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human BCAR3 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol BCAR3
Gene id 8412
Organism Homo sapiens
Official full symbol BCAR3 adaptor protein, NSP family member
Gene type protein-coding
Also known as AND-34, NSP2, SH2D3B
Summary Breast tumors are initially dependent on estrogens for growth and progression and can be inhibited by anti-estrogens such as tamoxifen. However, breast cancers progress to become anti-estrogen resistant. Breast cancer anti-estrogen resistance gene 3 was identified in the search for genes involved in the development of estrogen resistance. The gene encodes a component of intracellular signal transduction that causes estrogen-independent proliferation in human breast cancer cells. The protein contains a putative src homology 2 (SH2) domain, a hall mark of cellular tyrosine kinase signaling molecules, and is partly homologous to the cell division cycle protein CDC48. Multiple transcript variants encoding different isoforms have been found for this gene.
Genomic regions Chromosome 1
Strategy Summary
This gene has 5 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
BCAR3-201 ENST00000260502.11 3229 825aa Protein coding CCDS745 O75815-1 NM_003567.4 TSL:1, GENCODE basic, APPRIS P4, MANE Select v0.92,
BCAR3-203 ENST00000370244.5 3217 825aa Protein coding CCDS745 O75815-1 - TSL:1, GENCODE basic, APPRIS P4,
BCAR3-202 ENST00000370243.1 3200 825aa Protein coding CCDS745 O75815-1 - TSL:2, GENCODE basic, APPRIS P4,
BCAR3-204 ENST00000370247.7 2827 734aa Protein coding CCDS58010 O75815-3 - TSL:1, GENCODE basic, APPRIS ALT2,
BCAR3-211 ENST00000539242.5 2114 501aa Protein coding CCDS76181 O75815-2 - TSL:1, GENCODE basic,
BCAR3-205 ENST00000433544.1 1671 No protein Processed transcript - - - TSL:1,
BCAR3-212 ENST00000649852.1 1543 No protein Processed transcript - - - -
BCAR3-206 ENST00000466632.1 887 No protein Processed transcript - - - TSL:2,
BCAR3-207 ENST00000469315.1 490 No protein Processed transcript - - - TSL:2,
BCAR3-209 ENST00000490377.1 467 No protein Processed transcript - - - TSL:3,
BCAR3-208 ENST00000479503.2 321 No protein Processed transcript - - - TSL:2,
BCAR3-210 ENST00000538653.1 900 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene BCAR3
This KO Strategy loading
Red Cotton™ Notes Gene BCAR3 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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