CRISPR-U™ Gene Knockout Cell Line
Strategy
BCAR3 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human BCAR3 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | BCAR3 |
| Gene id | 8412 |
| Organism | Homo sapiens |
| Official full symbol | BCAR3 adaptor protein, NSP family member |
| Gene type | protein-coding |
| Also known as | AND-34, NSP2, SH2D3B |
| Summary | Breast tumors are initially dependent on estrogens for growth and progression and can be inhibited by anti-estrogens such as tamoxifen. However, breast cancers progress to become anti-estrogen resistant. Breast cancer anti-estrogen resistance gene 3 was identified in the search for genes involved in the development of estrogen resistance. The gene encodes a component of intracellular signal transduction that causes estrogen-independent proliferation in human breast cancer cells. The protein contains a putative src homology 2 (SH2) domain, a hall mark of cellular tyrosine kinase signaling molecules, and is partly homologous to the cell division cycle protein CDC48. Multiple transcript variants encoding different isoforms have been found for this gene. |
| Genomic regions | Chromosome 1 |
Strategy Summary
This gene has 5 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| BCAR3-201 | ENST00000260502.11 | 3229 | 825aa | Protein coding | CCDS745 | O75815-1 | NM_003567.4 | TSL:1, GENCODE basic, APPRIS P4, MANE Select v0.92, |
| BCAR3-203 | ENST00000370244.5 | 3217 | 825aa | Protein coding | CCDS745 | O75815-1 | - | TSL:1, GENCODE basic, APPRIS P4, |
| BCAR3-202 | ENST00000370243.1 | 3200 | 825aa | Protein coding | CCDS745 | O75815-1 | - | TSL:2, GENCODE basic, APPRIS P4, |
| BCAR3-204 | ENST00000370247.7 | 2827 | 734aa | Protein coding | CCDS58010 | O75815-3 | - | TSL:1, GENCODE basic, APPRIS ALT2, |
| BCAR3-211 | ENST00000539242.5 | 2114 | 501aa | Protein coding | CCDS76181 | O75815-2 | - | TSL:1, GENCODE basic, |
| BCAR3-205 | ENST00000433544.1 | 1671 | No protein | Processed transcript | - | - | - | TSL:1, |
| BCAR3-212 | ENST00000649852.1 | 1543 | No protein | Processed transcript | - | - | - | - |
| BCAR3-206 | ENST00000466632.1 | 887 | No protein | Processed transcript | - | - | - | TSL:2, |
| BCAR3-207 | ENST00000469315.1 | 490 | No protein | Processed transcript | - | - | - | TSL:2, |
| BCAR3-209 | ENST00000490377.1 | 467 | No protein | Processed transcript | - | - | - | TSL:3, |
| BCAR3-208 | ENST00000479503.2 | 321 | No protein | Processed transcript | - | - | - | TSL:2, |
| BCAR3-210 | ENST00000538653.1 | 900 | No protein | Retained intron | - | - | - | TSL:2, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | BCAR3 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene BCAR3 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
Comment: