CRISPR-U™ Gene Knockout Cell Line Strategy

SPOP Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human SPOP Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol SPOP
Gene id 8405
Organism Homo sapiens
Official full symbol speckle type BTB/POZ protein
Gene type protein-coding
Also known as BTBD32, NEDMACE, NEDMIDF, NSDVS1, NSDVS2, TEF2
Summary This gene encodes a protein that may modulate the transcriptional repression activities of death-associated protein 6 (DAXX), which interacts with histone deacetylase, core histones, and other histone-associated proteins. In mouse, the encoded protein binds to the putative leucine zipper domain of macroH2A1.2, a variant H2A histone that is enriched on inactivated X chromosomes. The BTB/POZ domain of this protein has been shown in other proteins to mediate transcriptional repression and to interact with components of histone deacetylase co-repressor complexes. Alternative splicing of this gene results in multiple transcript variants encoding the same protein.
Genomic regions Chromosome 17
Strategy Summary
This gene has 13 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
SPOP-202 ENST00000393328.6 2985 374aa Protein coding CCDS11551 O43791 - TSL:1, GENCODE basic, APPRIS P1,
SPOP-201 ENST00000347630.6 2965 374aa Protein coding CCDS11551 O43791 - TSL:5, GENCODE basic, APPRIS P1,
SPOP-206 ENST00000504102.6 2850 374aa Protein coding CCDS11551 O43791 NM_001007228.2 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
SPOP-224 ENST00000665825.1 2480 374aa Protein coding CCDS11551 O43791 - GENCODE basic, APPRIS P1,
SPOP-219 ENST00000514121.6 2414 374aa Protein coding CCDS11551 O43791 - TSL:4, GENCODE basic, APPRIS P1,
SPOP-205 ENST00000503676.5 1932 374aa Protein coding CCDS11551 O43791 - TSL:2, GENCODE basic, APPRIS P1,
SPOP-214 ENST00000509079.6 1860 374aa Protein coding CCDS11551 O43791 - TSL:5, GENCODE basic, APPRIS P1,
SPOP-209 ENST00000505581.5 984 238aa Protein coding - D6RDG8 - CDS 3' incomplete, TSL:5,
SPOP-212 ENST00000507970.5 919 185aa Protein coding - D6RGZ4 - CDS 3' incomplete, TSL:5,
SPOP-217 ENST00000510476.5 773 66aa Protein coding - D6RD94 - CDS 3' incomplete, TSL:3,
SPOP-220 ENST00000515508.6 563 117aa Protein coding - D6RFL7 - CDS 3' incomplete, TSL:2,
SPOP-203 ENST00000451526.6 450 32aa Protein coding - D6RA79 - CDS 3' incomplete, TSL:2,
SPOP-213 ENST00000508805.5 428 40aa Protein coding - D6RIS7 - CDS 3' incomplete, TSL:3,
SPOP-223 ENST00000659760.1 2081 165aa Nonsense mediated decay - A0A590UJA7 - -
SPOP-225 ENST00000671445.1 1716 66aa Nonsense mediated decay - D6RD94 - -
SPOP-218 ENST00000513080.6 1707 66aa Nonsense mediated decay - D6RD94 - TSL:4,
SPOP-210 ENST00000506399.5 918 44aa Nonsense mediated decay - D6RBR3 - TSL:5,
SPOP-216 ENST00000509869.5 779 72aa Nonsense mediated decay - H0Y977 - CDS 5' incomplete, TSL:5,
SPOP-207 ENST00000504212.5 534 44aa Nonsense mediated decay - D6RBR3 - TSL:4,
SPOP-204 ENST00000502385.1 574 No protein Processed transcript - - - TSL:4,
SPOP-221 ENST00000572686.1 2135 No protein Retained intron - - - TSL:NA,
SPOP-211 ENST00000507551.1 932 No protein Retained intron - - - TSL:3,
SPOP-215 ENST00000509765.1 613 No protein Retained intron - - - TSL:2,
SPOP-222 ENST00000577134.1 421 No protein Retained intron - - - TSL:2,
SPOP-208 ENST00000504889.5 400 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
Strategy
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene SPOP
This KO Strategy loading
Red Cotton™ Notes Gene SPOP had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
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