CRISPR-U™ Gene Knockout Cell Line Strategy

CASP7 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human CASP7 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol CASP7
Gene id 840
Organism Homo sapiens
Official full symbol caspase 7
Gene type protein-coding
Also known as CASP-7, CMH-1, ICE-LAP3, LICE2, MCH3
Summary This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. The precursor of the encoded protein is cleaved by caspase 3 and 10, is activated upon cell death stimuli and induces apoptosis. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
Genomic regions Chromosome 10
Strategy Summary
This gene has 11 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
CASP7-201 ENST00000345633.8 2659 303aa Protein coding CCDS7581 P55210-1 - TSL:1, GENCODE basic, APPRIS P1,
CASP7-204 ENST00000369321.6 2620 388aa Protein coding CCDS73200 A0A0A0MRL7 - TSL:2, GENCODE basic,
CASP7-210 ENST00000614447.4 2555 253aa Protein coding CCDS7582 P55210-2 - TSL:5, GENCODE basic,
CASP7-211 ENST00000621345.4 2467 303aa Protein coding CCDS7581 P55210-1 - TSL:1, GENCODE basic, APPRIS P1,
CASP7-202 ENST00000369315.5 2421 303aa Protein coding CCDS7581 P55210-1 - TSL:1, GENCODE basic, APPRIS P1,
CASP7-205 ENST00000369331.8 2380 253aa Protein coding CCDS7582 P55210-2 - TSL:1, GENCODE basic,
CASP7-212 ENST00000621607.4 2378 336aa Protein coding CCDS7580 P55210-3 - TSL:1, GENCODE basic,
CASP7-203 ENST00000369318.8 2344 303aa Protein coding CCDS7581 P55210-1 NM_001227.5 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
CASP7-207 ENST00000452490.3 2271 278aa Protein coding CCDS58096 P55210-4 - TSL:2, GENCODE basic,
CASP7-213 ENST00000672138.1 2351 302aa Protein coding - A0A5F9ZHZ6 - GENCODE basic,
CASP7-206 ENST00000429617.5 834 222aa Protein coding - Q5SVL2 - CDS 3' incomplete, TSL:3,
CASP7-208 ENST00000468790.1 607 No protein Processed transcript - - - TSL:2,
CASP7-209 ENST00000487232.1 799 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene CASP7
This KO Strategy loading
Red Cotton™ Notes Gene CASP7 had been KO in a549 cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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