CRISPR-U™ Gene Knockout Cell Line
Strategy
CASP7 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human CASP7 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | CASP7 |
| Gene id | 840 |
| Organism | Homo sapiens |
| Official full symbol | caspase 7 |
| Gene type | protein-coding |
| Also known as | CASP-7, CMH-1, ICE-LAP3, LICE2, MCH3 |
| Summary | This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. The precursor of the encoded protein is cleaved by caspase 3 and 10, is activated upon cell death stimuli and induces apoptosis. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. |
| Genomic regions | Chromosome 10 |
Strategy Summary
This gene has 11 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| CASP7-201 | ENST00000345633.8 | 2659 | 303aa | Protein coding | CCDS7581 | P55210-1 | - | TSL:1, GENCODE basic, APPRIS P1, |
| CASP7-204 | ENST00000369321.6 | 2620 | 388aa | Protein coding | CCDS73200 | A0A0A0MRL7 | - | TSL:2, GENCODE basic, |
| CASP7-210 | ENST00000614447.4 | 2555 | 253aa | Protein coding | CCDS7582 | P55210-2 | - | TSL:5, GENCODE basic, |
| CASP7-211 | ENST00000621345.4 | 2467 | 303aa | Protein coding | CCDS7581 | P55210-1 | - | TSL:1, GENCODE basic, APPRIS P1, |
| CASP7-202 | ENST00000369315.5 | 2421 | 303aa | Protein coding | CCDS7581 | P55210-1 | - | TSL:1, GENCODE basic, APPRIS P1, |
| CASP7-205 | ENST00000369331.8 | 2380 | 253aa | Protein coding | CCDS7582 | P55210-2 | - | TSL:1, GENCODE basic, |
| CASP7-212 | ENST00000621607.4 | 2378 | 336aa | Protein coding | CCDS7580 | P55210-3 | - | TSL:1, GENCODE basic, |
| CASP7-203 | ENST00000369318.8 | 2344 | 303aa | Protein coding | CCDS7581 | P55210-1 | NM_001227.5 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| CASP7-207 | ENST00000452490.3 | 2271 | 278aa | Protein coding | CCDS58096 | P55210-4 | - | TSL:2, GENCODE basic, |
| CASP7-213 | ENST00000672138.1 | 2351 | 302aa | Protein coding | - | A0A5F9ZHZ6 | - | GENCODE basic, |
| CASP7-206 | ENST00000429617.5 | 834 | 222aa | Protein coding | - | Q5SVL2 | - | CDS 3' incomplete, TSL:3, |
| CASP7-208 | ENST00000468790.1 | 607 | No protein | Processed transcript | - | - | - | TSL:2, |
| CASP7-209 | ENST00000487232.1 | 799 | No protein | Retained intron | - | - | - | TSL:2, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | CASP7 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene CASP7 had been KO in a549 cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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