CRISPR-U™ Gene Knockout Cell Line
Strategy
ANGPTL6 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human ANGPTL6 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | ANGPTL6 |
| Gene id | 83854 |
| Organism | Homo sapiens |
| Official full symbol | angiopoietin like 6 |
| Gene type | protein-coding |
| Also known as | AGF, ARP5 |
| Genomic regions | Chromosome 19 |
Strategy Summary
This gene has 3 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| ANGPTL6-201 | ENST00000253109.4 | 1900 | 470aa | Protein coding | CCDS12224 | A0A024R7A9 Q8NI99 | - | TSL:1, GENCODE basic, APPRIS P1, |
| ANGPTL6-204 | ENST00000592641.5 | 1781 | 470aa | Protein coding | CCDS12224 | A0A024R7A9 Q8NI99 | - | TSL:1, GENCODE basic, APPRIS P1, |
| ANGPTL6-203 | ENST00000589181.5 | 1579 | 430aa | Protein coding | - | K7EKF6 | - | TSL:5, GENCODE basic, |
| ANGPTL6-202 | ENST00000586910.1 | 568 | No protein | Retained intron | - | - | - | TSL:4, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | ANGPTL6 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene ANGPTL6 had been KO in a549 cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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