CRISPR-U™ Gene Knockout Cell Line Strategy

ATG10 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human ATG10 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol ATG10
Gene id 83734
Organism Homo sapiens
Official full symbol autophagy related 10
Gene type protein-coding
Also known as APG10, APG10L, pp12616
Summary Autophagy is a process for the bulk degradation of cytosolic compartments by lysosomes. ATG10 is an E2-like enzyme involved in 2 ubiquitin-like modifications essential for autophagosome formation: ATG12 (MIM 609608)-ATG5 (MIM 604261) conjugation and modification of a soluble form of MAP-LC3 (MAP1LC3A; MIM 601242), a homolog of yeast Apg8, to a membrane-bound form (Nemoto et al., 2003 ).
Genomic regions Chromosome 5
Strategy Summary
This gene has 6 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
ATG10-201 ENST00000282185.8 3029 220aa Protein coding CCDS4057 Q9H0Y0-1 NM_031482.5 TSL:1, GENCODE basic, APPRIS P2, MANE Select v0.92,
ATG10-203 ENST00000458350.7 1795 220aa Protein coding CCDS4057 Q9H0Y0-1 - TSL:1, GENCODE basic, APPRIS P2,
ATG10-202 ENST00000355178.8 1438 125aa Protein coding - Q9H0Y0-2 - TSL:2, GENCODE basic,
ATG10-207 ENST00000513443.5 978 125aa Protein coding - Q9H0Y0-2 - TSL:1, GENCODE basic,
ATG10-208 ENST00000513634.1 618 184aa Protein coding - D6RDX3 - TSL:2, GENCODE basic, APPRIS ALT2,
ATG10-206 ENST00000510085.1 549 36aa Protein coding - D6RC89 - CDS 3' incomplete, TSL:4,
ATG10-204 ENST00000504770.5 813 171aa Nonsense mediated decay - D6RBD1 - TSL:3,
ATG10-209 ENST00000514253.2 904 No protein Processed transcript - - - TSL:3,
ATG10-205 ENST00000508814.5 413 No protein Processed transcript - - - TSL:3,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene ATG10
This KO Strategy loading
Red Cotton™ Notes Gene ATG10 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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