CRISPR-U™ Gene Knockout Cell Line
Strategy
ATG10 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human ATG10 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | ATG10 |
| Gene id | 83734 |
| Organism | Homo sapiens |
| Official full symbol | autophagy related 10 |
| Gene type | protein-coding |
| Also known as | APG10, APG10L, pp12616 |
| Summary | Autophagy is a process for the bulk degradation of cytosolic compartments by lysosomes. ATG10 is an E2-like enzyme involved in 2 ubiquitin-like modifications essential for autophagosome formation: ATG12 (MIM 609608)-ATG5 (MIM 604261) conjugation and modification of a soluble form of MAP-LC3 (MAP1LC3A; MIM 601242), a homolog of yeast Apg8, to a membrane-bound form (Nemoto et al., 2003 ). |
| Genomic regions | Chromosome 5 |
Strategy Summary
This gene has 6 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| ATG10-201 | ENST00000282185.8 | 3029 | 220aa | Protein coding | CCDS4057 | Q9H0Y0-1 | NM_031482.5 | TSL:1, GENCODE basic, APPRIS P2, MANE Select v0.92, |
| ATG10-203 | ENST00000458350.7 | 1795 | 220aa | Protein coding | CCDS4057 | Q9H0Y0-1 | - | TSL:1, GENCODE basic, APPRIS P2, |
| ATG10-202 | ENST00000355178.8 | 1438 | 125aa | Protein coding | - | Q9H0Y0-2 | - | TSL:2, GENCODE basic, |
| ATG10-207 | ENST00000513443.5 | 978 | 125aa | Protein coding | - | Q9H0Y0-2 | - | TSL:1, GENCODE basic, |
| ATG10-208 | ENST00000513634.1 | 618 | 184aa | Protein coding | - | D6RDX3 | - | TSL:2, GENCODE basic, APPRIS ALT2, |
| ATG10-206 | ENST00000510085.1 | 549 | 36aa | Protein coding | - | D6RC89 | - | CDS 3' incomplete, TSL:4, |
| ATG10-204 | ENST00000504770.5 | 813 | 171aa | Nonsense mediated decay | - | D6RBD1 | - | TSL:3, |
| ATG10-209 | ENST00000514253.2 | 904 | No protein | Processed transcript | - | - | - | TSL:3, |
| ATG10-205 | ENST00000508814.5 | 413 | No protein | Processed transcript | - | - | - | TSL:3, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | ATG10 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene ATG10 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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