CRISPR-U™ Gene Knockout Cell Line Strategy

SESN2 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human SESN2 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol SESN2
Gene id 83667
Organism Homo sapiens
Official full symbol sestrin 2
Gene type protein-coding
Also known as HI95, SES2, SEST2
Summary This gene encodes a member of the sestrin family of PA26-related proteins. The encoded protein may function in the regulation of cell growth and survival. This protein may be involved in cellular response to different stress conditions.
Genomic regions Chromosome 1
Strategy Summary
This gene has 1 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
SESN2-201 ENST00000253063.4 3462 480aa Protein coding CCDS321 P58004 NM_031459.5 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
Ubigene Red Cotton Transcript
Strategy
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene SESN2
This KO Strategy loading
Red Cotton™ Notes Gene SESN2 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:

$49

Single gRNA plasmid off-shelf

$599

Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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