CRISPR-U™ Gene Knockout Cell Line
Strategy
SESN2 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human SESN2 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | SESN2 |
| Gene id | 83667 |
| Organism | Homo sapiens |
| Official full symbol | sestrin 2 |
| Gene type | protein-coding |
| Also known as | HI95, SES2, SEST2 |
| Summary | This gene encodes a member of the sestrin family of PA26-related proteins. The encoded protein may function in the regulation of cell growth and survival. This protein may be involved in cellular response to different stress conditions. |
| Genomic regions | Chromosome 1 |
Strategy Summary
This gene has 1 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| SESN2-201 | ENST00000253063.4 | 3462 | 480aa | Protein coding | CCDS321 | P58004 | NM_031459.5 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | SESN2 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene SESN2 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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