CRISPR-U™ Gene Knockout Cell Line
Strategy
PARP9 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human PARP9 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | PARP9 |
| Gene id | 83666 |
| Organism | Homo sapiens |
| Official full symbol | poly(ADP-ribose) polymerase family member 9 |
| Gene type | protein-coding |
| Also known as | ARTD9, BAL, BAL1, MGC:7868 |
| Genomic regions | Chromosome 3 |
Strategy Summary
This gene has 6 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| PARP9-202 | ENST00000462315.5 | 6769 | 710aa | Protein coding | CCDS54633 | Q8IXQ6-3 | - | TSL:1, GENCODE basic, |
| PARP9-201 | ENST00000360356.6 | 3198 | 854aa | Protein coding | CCDS3014 | Q8IXQ6-1 | - | TSL:1, GENCODE basic, APPRIS P4, |
| PARP9-205 | ENST00000477522.6 | 3128 | 819aa | Protein coding | CCDS54634 | Q8IXQ6-2 | - | TSL:1, GENCODE basic, APPRIS ALT2, |
| PARP9-204 | ENST00000471785.5 | 3040 | 819aa | Protein coding | CCDS54634 | Q8IXQ6-2 | - | TSL:2, GENCODE basic, APPRIS ALT2, |
| PARP9-207 | ENST00000492382.5 | 1837 | 399aa | Protein coding | - | G5E9U8 | - | TSL:1, GENCODE basic, |
| PARP9-203 | ENST00000466126.1 | 567 | 115aa | Protein coding | - | C9K0E5 | - | CDS 3' incomplete, TSL:4, |
| PARP9-206 | ENST00000489652.1 | 2642 | No protein | Retained intron | - | - | - | TSL:2, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | PARP9 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene PARP9 had been KO in a549 cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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