CRISPR-U™ Gene Knockout Cell Line Strategy

PARP9 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human PARP9 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol PARP9
Gene id 83666
Organism Homo sapiens
Official full symbol poly(ADP-ribose) polymerase family member 9
Gene type protein-coding
Also known as ARTD9, BAL, BAL1, MGC:7868
Genomic regions Chromosome 3
Strategy Summary
This gene has 6 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
PARP9-202 ENST00000462315.5 6769 710aa Protein coding CCDS54633 Q8IXQ6-3 - TSL:1, GENCODE basic,
PARP9-201 ENST00000360356.6 3198 854aa Protein coding CCDS3014 Q8IXQ6-1 - TSL:1, GENCODE basic, APPRIS P4,
PARP9-205 ENST00000477522.6 3128 819aa Protein coding CCDS54634 Q8IXQ6-2 - TSL:1, GENCODE basic, APPRIS ALT2,
PARP9-204 ENST00000471785.5 3040 819aa Protein coding CCDS54634 Q8IXQ6-2 - TSL:2, GENCODE basic, APPRIS ALT2,
PARP9-207 ENST00000492382.5 1837 399aa Protein coding - G5E9U8 - TSL:1, GENCODE basic,
PARP9-203 ENST00000466126.1 567 115aa Protein coding - C9K0E5 - CDS 3' incomplete, TSL:4,
PARP9-206 ENST00000489652.1 2642 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene PARP9
This KO Strategy loading
Red Cotton™ Notes Gene PARP9 had been KO in a549 cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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