CRISPR-U™ Gene Knockout Cell Line Strategy

CASP1 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human CASP1 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol CASP1
Gene id 834
Organism Homo sapiens
Official full symbol caspase 1
Gene type protein-coding
Also known as ICE, IL1BC, P45
Summary This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme. This gene was identified by its ability to proteolytically cleave and activate the inactive precursor of interleukin-1, a cytokine involved in the processes such as inflammation, septic shock, and wound healing. This gene has been shown to induce cell apoptosis and may function in various developmental stages. Studies of a similar gene in mouse suggest a role in the pathogenesis of Huntington disease. Alternative splicing results in transcript variants encoding distinct isoforms.
Genomic regions Chromosome 11
Strategy Summary
This gene has 12 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
CASP1-215 ENST00000533400.6 1996 404aa Protein coding CCDS8330 P29466-1 NM_001257118.3 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
CASP1-202 ENST00000436863.7 1477 404aa Protein coding CCDS8330 P29466-1 - TSL:1, GENCODE basic, APPRIS P1,
CASP1-204 ENST00000525825.5 1237 383aa Protein coding CCDS8329 P29466-2 - TSL:2, GENCODE basic,
CASP1-206 ENST00000526568.5 1061 311aa Protein coding CCDS53704 P29466-3 - TSL:1, GENCODE basic,
CASP1-203 ENST00000446369.5 948 263aa Protein coding CCDS8331 P29466-4 - TSL:1, GENCODE basic,
CASP1-216 ENST00000534497.5 825 263aa Protein coding CCDS8331 P29466-4 - TSL:5, GENCODE basic,
CASP1-201 ENST00000353247.9 423 88aa Protein coding CCDS8332 P29466-5 - TSL:1, GENCODE basic,
CASP1-212 ENST00000531166.5 300 88aa Protein coding CCDS8332 P29466-5 - TSL:5, GENCODE basic,
CASP1-208 ENST00000527979.5 1521 367aa Protein coding - G3V169 - TSL:5, GENCODE basic,
CASP1-210 ENST00000528974.1 1402 301aa Protein coding - B4DVD8 - TSL:2, GENCODE basic,
CASP1-213 ENST00000532439.5 821 236aa Protein coding - H0YEC7 - CDS 5' incomplete, TSL:5,
CASP1-217 ENST00000640184.1 327 108aa Protein coding - A0A1W2PRX7 - TSL:1, GENCODE basic,
CASP1-211 ENST00000529871.1 1392 110aa Nonsense mediated decay - Q5FBZ0 - TSL:1,
CASP1-205 ENST00000526511.5 1202 No protein Retained intron - - - TSL:2,
CASP1-209 ENST00000528424.1 578 No protein Retained intron - - - TSL:4,
CASP1-214 ENST00000532520.1 525 No protein Retained intron - - - TSL:2,
CASP1-207 ENST00000527625.5 424 No protein Retained intron - - - TSL:3,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene CASP1
This KO Strategy loading
Red Cotton™ Notes Gene CASP1 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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