CRISPR-U™ Gene Knockout Cell Line
Strategy
CASP1 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human CASP1 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | CASP1 |
| Gene id | 834 |
| Organism | Homo sapiens |
| Official full symbol | caspase 1 |
| Gene type | protein-coding |
| Also known as | ICE, IL1BC, P45 |
| Summary | This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme. This gene was identified by its ability to proteolytically cleave and activate the inactive precursor of interleukin-1, a cytokine involved in the processes such as inflammation, septic shock, and wound healing. This gene has been shown to induce cell apoptosis and may function in various developmental stages. Studies of a similar gene in mouse suggest a role in the pathogenesis of Huntington disease. Alternative splicing results in transcript variants encoding distinct isoforms. |
| Genomic regions | Chromosome 11 |
Strategy Summary
This gene has 12 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| CASP1-215 | ENST00000533400.6 | 1996 | 404aa | Protein coding | CCDS8330 | P29466-1 | NM_001257118.3 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| CASP1-202 | ENST00000436863.7 | 1477 | 404aa | Protein coding | CCDS8330 | P29466-1 | - | TSL:1, GENCODE basic, APPRIS P1, |
| CASP1-204 | ENST00000525825.5 | 1237 | 383aa | Protein coding | CCDS8329 | P29466-2 | - | TSL:2, GENCODE basic, |
| CASP1-206 | ENST00000526568.5 | 1061 | 311aa | Protein coding | CCDS53704 | P29466-3 | - | TSL:1, GENCODE basic, |
| CASP1-203 | ENST00000446369.5 | 948 | 263aa | Protein coding | CCDS8331 | P29466-4 | - | TSL:1, GENCODE basic, |
| CASP1-216 | ENST00000534497.5 | 825 | 263aa | Protein coding | CCDS8331 | P29466-4 | - | TSL:5, GENCODE basic, |
| CASP1-201 | ENST00000353247.9 | 423 | 88aa | Protein coding | CCDS8332 | P29466-5 | - | TSL:1, GENCODE basic, |
| CASP1-212 | ENST00000531166.5 | 300 | 88aa | Protein coding | CCDS8332 | P29466-5 | - | TSL:5, GENCODE basic, |
| CASP1-208 | ENST00000527979.5 | 1521 | 367aa | Protein coding | - | G3V169 | - | TSL:5, GENCODE basic, |
| CASP1-210 | ENST00000528974.1 | 1402 | 301aa | Protein coding | - | B4DVD8 | - | TSL:2, GENCODE basic, |
| CASP1-213 | ENST00000532439.5 | 821 | 236aa | Protein coding | - | H0YEC7 | - | CDS 5' incomplete, TSL:5, |
| CASP1-217 | ENST00000640184.1 | 327 | 108aa | Protein coding | - | A0A1W2PRX7 | - | TSL:1, GENCODE basic, |
| CASP1-211 | ENST00000529871.1 | 1392 | 110aa | Nonsense mediated decay | - | Q5FBZ0 | - | TSL:1, |
| CASP1-205 | ENST00000526511.5 | 1202 | No protein | Retained intron | - | - | - | TSL:2, |
| CASP1-209 | ENST00000528424.1 | 578 | No protein | Retained intron | - | - | - | TSL:4, |
| CASP1-214 | ENST00000532520.1 | 525 | No protein | Retained intron | - | - | - | TSL:2, |
| CASP1-207 | ENST00000527625.5 | 424 | No protein | Retained intron | - | - | - | TSL:3, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | CASP1 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene CASP1 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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