CRISPR-U™ Gene Knockout Cell Line Strategy

BRAP Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human BRAP Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol BRAP
Gene id 8315
Organism Homo sapiens
Official full symbol BRCA1 associated protein
Gene type protein-coding
Also known as BRAP2, IMP, RNF52
Summary The protein encoded by this gene was identified by its ability to bind to the nuclear localization signal of BRCA1 and other proteins. It is a cytoplasmic protein which may regulate nuclear targeting by retaining proteins with a nuclear localization signal in the cytoplasm.
Genomic regions Chromosome 12
Strategy Summary
This gene has 2 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
BRAP-202 ENST00000419234.9 3996 592aa Protein coding CCDS9154 Q7Z569 NM_006768.5 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
BRAP-201 ENST00000327551.6 1947 562aa Protein coding - J3KNN7 - TSL:1, GENCODE basic,
BRAP-203 ENST00000547043.1 1543 No protein Retained intron - - - TSL:3,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene BRAP
This KO Strategy loading
Red Cotton™ Notes Gene BRAP had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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