CRISPR-U™ Gene Knockout Cell Line
Strategy
BRAP Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human BRAP Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | BRAP |
| Gene id | 8315 |
| Organism | Homo sapiens |
| Official full symbol | BRCA1 associated protein |
| Gene type | protein-coding |
| Also known as | BRAP2, IMP, RNF52 |
| Summary | The protein encoded by this gene was identified by its ability to bind to the nuclear localization signal of BRCA1 and other proteins. It is a cytoplasmic protein which may regulate nuclear targeting by retaining proteins with a nuclear localization signal in the cytoplasm. |
| Genomic regions | Chromosome 12 |
Strategy Summary
This gene has 2 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| BRAP-202 | ENST00000419234.9 | 3996 | 592aa | Protein coding | CCDS9154 | Q7Z569 | NM_006768.5 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| BRAP-201 | ENST00000327551.6 | 1947 | 562aa | Protein coding | - | J3KNN7 | - | TSL:1, GENCODE basic, |
| BRAP-203 | ENST00000547043.1 | 1543 | No protein | Retained intron | - | - | - | TSL:3, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | BRAP |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene BRAP had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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