CRISPR-U™ Gene Knockout Cell Line Strategy

CAST Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human CAST Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol CAST
Gene id 831
Organism Homo sapiens
Official full symbol calpastatin
Gene type protein-coding
Also known as BS-17, PLACK
Summary The protein encoded by this gene is an endogenous calpain (calcium-dependent cysteine protease) inhibitor. It consists of an N-terminal domain L and four repetitive calpain-inhibition domains (domains 1-4), and it is involved in the proteolysis of amyloid precursor protein. The calpain/calpastatin system is involved in numerous membrane fusion events, such as neural vesicle exocytosis and platelet and red-cell aggregation. The encoded protein is also thought to affect the expression levels of genes encoding structural or regulatory proteins. Alternatively spliced transcript variants encoding different isoforms have been described.
Genomic regions Chromosome 5
Strategy Summary
This gene has 38 protein coding transcripts：
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
CAST-206 ENST00000395812.6 4506 750aa Protein coding CCDS54882 P20810-9 - TSL:5, GENCODE basic, APPRIS P4,
CAST-247 ENST00000674984.1 4330 769aa Protein coding CCDS43344 - - GENCODE basic, APPRIS ALT2,
CAST-201 ENST00000309190.9 4282 686aa Protein coding CCDS4082 P20810-8 - TSL:1, GENCODE basic, APPRIS ALT2,
CAST-203 ENST00000338252.7 2972 695aa Protein coding CCDS54883 P20810-2 - TSL:1, GENCODE basic,
CAST-213 ENST00000504465.5 2336 636aa Protein coding CCDS75279 E9PDE4 - TSL:2, GENCODE basic, APPRIS ALT2,
CAST-228 ENST00000509903.5 2171 673aa Protein coding CCDS83017 E9PCH5 - TSL:1, GENCODE basic,
CAST-250 ENST00000675179.1 4490 791aa Protein coding - - NM_001750.7 GENCODE basic, APPRIS ALT2, MANE Select v0.92,
CAST-207 ENST00000395813.5 4356 708aa Protein coding - P20810-1 - TSL:5, GENCODE basic, APPRIS ALT2,
CAST-248 ENST00000675033.1 4296 667aa Protein coding - - - GENCODE basic, APPRIS ALT2,
CAST-257 ENST00000675663.1 4263 754aa Protein coding - - - GENCODE basic, APPRIS ALT2,
CAST-246 ENST00000674702.1 4067 704aa Protein coding - - - GENCODE basic, APPRIS ALT2,
CAST-245 ENST00000674587.1 4024 609aa Protein coding - - - GENCODE basic,
CAST-255 ENST00000675479.1 3991 662aa Protein coding - - - GENCODE basic,
CAST-209 ENST00000437034.6 3377 436aa Protein coding - H0Y7F0 - CDS 5' incomplete, TSL:1,
CAST-202 ENST00000325674.11 3356 430aa Protein coding - A0A0A0MR45 - TSL:5, GENCODE basic,
CAST-234 ENST00000511049.5 3273 693aa Protein coding - E7EVY3 - TSL:5, GENCODE basic, APPRIS ALT2,
CAST-251 ENST00000675185.1 2772 252aa Protein coding - - - CDS 5' incomplete,
CAST-256 ENST00000675614.1 2727 681aa Protein coding - - - GENCODE basic,
CAST-223 ENST00000508608.6 2699 741aa Protein coding - A0A0C4DGB5 - TSL:2, GENCODE basic,
CAST-224 ENST00000508830.5 2539 791aa Protein coding - P20810-6 - TSL:5, GENCODE basic, APPRIS ALT2,
CAST-259 ENST00000675858.1 2529 615aa Protein coding - - - GENCODE basic,
CAST-204 ENST00000341926.7 2487 708aa Protein coding - P20810-1 - TSL:1, GENCODE basic, APPRIS ALT2,
CAST-236 ENST00000511782.5 2466 694aa Protein coding - B7Z574 - TSL:2, GENCODE basic, APPRIS ALT2,
CAST-233 ENST00000510756.6 2428 667aa Protein coding - P20810-10 - TSL:5, GENCODE basic, APPRIS ALT2,
CAST-230 ENST00000510156.5 2079 642aa Protein coding - E7ES10 - CDS 3' incomplete, TSL:5,
CAST-232 ENST00000510500.5 1835 465aa Protein coding - H0Y9H6 - CDS 5' incomplete, TSL:1,
CAST-222 ENST00000508579.5 1758 423aa Protein coding - E7EQ12 - TSL:2, GENCODE basic,
CAST-244 ENST00000515663.5 1473 431aa Protein coding - E7EQA0 - TSL:2, GENCODE basic,
CAST-235 ENST00000511097.6 1399 393aa Protein coding - F8W7E0 - CDS 3' incomplete, TSL:5,
CAST-208 ENST00000421689.6 913 304aa Protein coding - E9PSG1 - CDS 3' incomplete, TSL:5,
CAST-238 ENST00000512620.5 904 301aa Protein coding - H0YA91 - CDS 5' and 3' incomplete, TSL:5,
CAST-215 ENST00000505143.5 864 288aa Protein coding - H0Y944 - CDS 5' and 3' incomplete, TSL:3,
CAST-241 ENST00000514845.5 588 150aa Protein coding - D6RBR1 - CDS 3' incomplete, TSL:4,
CAST-217 ENST00000506811.5 582 156aa Protein coding - E7EN75 - CDS 3' incomplete, TSL:5,
CAST-221 ENST00000508197.1 567 156aa Protein coding - E7EQK6 - CDS 3' incomplete, TSL:3,
CAST-225 ENST00000509259.5 565 91aa Protein coding - D6RC54 - CDS 3' incomplete, TSL:4,
CAST-240 ENST00000514055.5 559 149aa Protein coding - D6RGF7 - CDS 3' incomplete, TSL:5,
CAST-211 ENST00000503828.5 499 78aa Protein coding - D6RAA8 - CDS 3' incomplete, TSL:2,
CAST-252 ENST00000675266.1 4323 81aa Nonsense mediated decay - - - CDS 5' incomplete,
CAST-253 ENST00000675267.1 4300 110aa Nonsense mediated decay - - - -
CAST-210 ENST00000484552.6 1767 430aa Nonsense mediated decay - H0YD33 - TSL:1,
CAST-254 ENST00000675275.1 1399 94aa Nonsense mediated decay - - - CDS 5' incomplete,
CAST-229 ENST00000510098.1 1323 203aa Nonsense mediated decay - A0A0C4DGD1 - TSL:1,
CAST-220 ENST00000508117.6 571 82aa Nonsense mediated decay - D6RBZ8 - TSL:5,
CAST-205 ENST00000348386.7 2272 No protein Processed transcript - - - TSL:1,
CAST-258 ENST00000675734.1 1111 No protein Processed transcript - - - -
CAST-212 ENST00000504221.5 578 No protein Processed transcript - - - TSL:3,
CAST-227 ENST00000509602.1 557 No protein Processed transcript - - - TSL:4,
CAST-219 ENST00000507836.1 449 No protein Processed transcript - - - TSL:3,
CAST-218 ENST00000507487.5 396 No protein Processed transcript - - - TSL:5,
CAST-216 ENST00000505402.1 362 No protein Processed transcript - - - TSL:4,
CAST-249 ENST00000675107.1 3117 No protein Retained intron - - - -
CAST-226 ENST00000509529.1 3066 No protein Retained intron - - - TSL:2,
CAST-239 ENST00000513666.5 1539 No protein Retained intron - - - TSL:2,
CAST-214 ENST00000504532.1 714 No protein Retained intron - - - TSL:2,
CAST-242 ENST00000515063.1 654 No protein Retained intron - - - TSL:1,
CAST-243 ENST00000515160.5 617 No protein Retained intron - - - TSL:2,
CAST-231 ENST00000510245.1 561 No protein Retained intron - - - TSL:4,
CAST-237 ENST00000512191.1 406 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene CAST
This KO Strategy loading
Red Cotton™ Notes Gene CAST had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
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