CRISPR-U™ Gene Knockout Cell Line Strategy

PICALM Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human PICALM Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol PICALM
Gene id 8301
Organism Homo sapiens
Official full symbol phosphatidylinositol binding clathrin assembly protein
Gene type protein-coding
Also known as CALM, CLTH, LAP
Summary This gene encodes a clathrin assembly protein, which recruits clathrin and adaptor protein complex 2 (AP2) to cell membranes at sites of coated-pit formation and clathrin-vesicle assembly. The protein may be required to determine the amount of membrane to be recycled, possibly by regulating the size of the clathrin cage. The protein is involved in AP2-dependent clathrin-mediated endocytosis at the neuromuscular junction. A chromosomal translocation t(10;11)(p13;q14) leading to the fusion of this gene and the MLLT10 gene is found in acute lymphoblastic leukemia, acute myeloid leukemia and malignant lymphomas. The polymorphisms of this gene are associated with the risk of Alzheimer disease. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Genomic regions Chromosome 11
Strategy Summary
This gene has 15 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
PICALM-202 ENST00000393346.8 4134 652aa Protein coding CCDS8272 Q13492-1 NM_007166.4 TSL:1, GENCODE basic, APPRIS P4, MANE Select v0.92,
PICALM-204 ENST00000526033.5 3613 645aa Protein coding CCDS55784 Q13492-5 - TSL:1, GENCODE basic,
PICALM-219 ENST00000532317.5 3474 610aa Protein coding CCDS31653 Q13492-3 - TSL:1, GENCODE basic, APPRIS ALT1,
PICALM-209 ENST00000528398.5 2049 551aa Protein coding CCDS55783 Q13492-4 - TSL:2, GENCODE basic,
PICALM-224 ENST00000630913.2 2261 105aa Protein coding - E9PK13 - TSL:5, GENCODE basic,
PICALM-201 ENST00000356360.9 1958 632aa Protein coding - Q13492-2 - TSL:5, GENCODE basic,
PICALM-212 ENST00000529760.5 1153 308aa Protein coding - H0YEH1 - CDS 5' incomplete, TSL:5,
PICALM-214 ENST00000530692.5 963 215aa Protein coding - H0YEY8 - CDS 5' incomplete, TSL:5,
PICALM-213 ENST00000530542.1 905 302aa Protein coding - H0YEF7 - CDS 5' and 3' incomplete, TSL:5,
PICALM-207 ENST00000526961.5 802 264aa Protein coding - H0YD48 - CDS 5' incomplete, TSL:5,
PICALM-211 ENST00000529016.5 757 244aa Protein coding - H0YCY1 - CDS 5' incomplete, TSL:3,
PICALM-217 ENST00000531930.5 703 185aa Protein coding - E9PLJ8 - CDS 3' incomplete, TSL:5,
PICALM-220 ENST00000532603.5 566 134aa Protein coding - H0YE97 - CDS 5' incomplete, TSL:4,
PICALM-208 ENST00000528256.1 563 148aa Protein coding - E9PKP6 - CDS 3' incomplete, TSL:4,
PICALM-203 ENST00000525162.5 548 147aa Protein coding - E9PI56 - CDS 3' incomplete, TSL:4,
PICALM-223 ENST00000534412.5 811 48aa Nonsense mediated decay - E9PJT1 - TSL:5,
PICALM-218 ENST00000532041.5 699 105aa Nonsense mediated decay - E9PK13 - TSL:3,
PICALM-215 ENST00000531558.1 565 48aa Nonsense mediated decay - E9PJT1 - TSL:5,
PICALM-210 ENST00000528411.1 586 No protein Processed transcript - - - TSL:5,
PICALM-205 ENST00000526548.3 738 No protein Retained intron - - - TSL:1,
PICALM-206 ENST00000526907.1 737 No protein Retained intron - - - TSL:5,
PICALM-216 ENST00000531771.5 631 No protein Retained intron - - - TSL:3,
PICALM-221 ENST00000533350.1 541 No protein Retained intron - - - TSL:3,
PICALM-222 ENST00000534375.1 390 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene PICALM
This KO Strategy loading
Red Cotton™ Notes Gene PICALM had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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