CRISPR-U™ Gene Knockout Cell Line Strategy

KDM5C Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human KDM5C Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol KDM5C
Gene id 8242
Organism Homo sapiens
Official full symbol lysine demethylase 5C
Gene type protein-coding
Also known as DXS1272E, JARID1C, MRX13, MRXJ, MRXSCJ, MRXSJ, SMCX, XE169
Summary This gene is a member of the SMCY homolog family and encodes a protein with one ARID domain, one JmjC domain, one JmjN domain and two PHD-type zinc fingers. The DNA-binding motifs suggest this protein is involved in the regulation of transcription and chromatin remodeling. Mutations in this gene have been associated with X-linked cognitive disability. Alternative splicing results in multiple transcript variants.
Genomic regions Chromosome X
Strategy Summary
This gene has 5 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
KDM5C-208 ENST00000452825.7 6096 1379aa Protein coding CCDS55417 P41229-4 - TSL:5, GENCODE basic,
KDM5C-204 ENST00000375401.8 5810 1560aa Protein coding CCDS14351 P41229-1 NM_004187.5 TSL:1, GENCODE basic, APPRIS P4, MANE Select v0.92,
KDM5C-205 ENST00000404049.7 5677 1559aa Protein coding CCDS65269 P41229-5 - TSL:1, GENCODE basic, APPRIS ALT2,
KDM5C-202 ENST00000375379.7 5245 1557aa Protein coding CCDS87747 P41229-2 - TSL:5, GENCODE basic, APPRIS ALT2,
KDM5C-203 ENST00000375383.7 5122 1516aa Protein coding - P41229-3 - TSL:5, GENCODE basic,
KDM5C-207 ENST00000429877.5 870 53aa Nonsense mediated decay - F8WDK1 - TSL:3,
KDM5C-206 ENST00000428012.1 766 99aa Nonsense mediated decay - F8WF56 - TSL:3,
KDM5C-201 ENST00000349663.6 548 74aa Nonsense mediated decay - F8W7H7 - TSL:4,
KDM5C-209 ENST00000465402.1 720 No protein Processed transcript - - - TSL:3,
KDM5C-213 ENST00000495519.1 600 No protein Processed transcript - - - TSL:3,
KDM5C-210 ENST00000467093.1 446 No protein Processed transcript - - - TSL:3,
KDM5C-211 ENST00000477109.1 297 No protein Processed transcript - - - TSL:3,
KDM5C-214 ENST00000497100.5 1403 No protein Retained intron - - - TSL:5,
KDM5C-212 ENST00000481369.1 1361 No protein Retained intron - - - TSL:2,
KDM5C-215 ENST00000497995.1 394 No protein Retained intron - - - TSL:3,
Ubigene Red Cotton Transcript
Strategy
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene KDM5C
This KO Strategy loading
Red Cotton™ Notes Gene KDM5C had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
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Work flow
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