CRISPR-U™ Gene Knockout Cell Line Strategy

USP9X Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human USP9X Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol USP9X
Gene id 8239
Organism Homo sapiens
Official full symbol ubiquitin specific peptidase 9 X-linked
Gene type protein-coding
Also known as DFFRX, FAF, FAM, MRX99, MRXS99F
Summary This gene is a member of the peptidase C19 family and encodes a protein that is similar to ubiquitin-specific proteases. Though this gene is located on the X chromosome, it escapes X-inactivation. Mutations in this gene have been associated with Turner syndrome. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
Genomic regions Chromosome X
Strategy Summary
This gene has 2 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
USP9X-202 ENST00000378308.7 12543 2554aa Protein coding CCDS55403 Q93008-1 NM_001039591.3 TSL:5, GENCODE basic, APPRIS P1, MANE Select v0.92,
USP9X-201 ENST00000324545.8 12401 2570aa Protein coding CCDS43930 Q93008-3 - TSL:5, GENCODE basic,
USP9X-203 ENST00000462850.1 548 No protein Processed transcript - - - TSL:4,
USP9X-207 ENST00000485180.1 757 No protein Retained intron - - - TSL:2,
USP9X-206 ENST00000467173.1 669 No protein Retained intron - - - TSL:3,
USP9X-204 ENST00000463829.1 666 No protein Retained intron - - - TSL:3,
USP9X-208 ENST00000487625.1 581 No protein Retained intron - - - TSL:2,
USP9X-205 ENST00000465386.1 479 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene USP9X
This KO Strategy loading
Red Cotton™ Notes Gene USP9X had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

Please leave your suggestion ×