CRISPR-U™ Gene Knockout Cell Line
Strategy
USP9X Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human USP9X Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | USP9X |
| Gene id | 8239 |
| Organism | Homo sapiens |
| Official full symbol | ubiquitin specific peptidase 9 X-linked |
| Gene type | protein-coding |
| Also known as | DFFRX, FAF, FAM, MRX99, MRXS99F |
| Summary | This gene is a member of the peptidase C19 family and encodes a protein that is similar to ubiquitin-specific proteases. Though this gene is located on the X chromosome, it escapes X-inactivation. Mutations in this gene have been associated with Turner syndrome. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. |
| Genomic regions | Chromosome X |
Strategy Summary
This gene has 2 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| USP9X-202 | ENST00000378308.7 | 12543 | 2554aa | Protein coding | CCDS55403 | Q93008-1 | NM_001039591.3 | TSL:5, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| USP9X-201 | ENST00000324545.8 | 12401 | 2570aa | Protein coding | CCDS43930 | Q93008-3 | - | TSL:5, GENCODE basic, |
| USP9X-203 | ENST00000462850.1 | 548 | No protein | Processed transcript | - | - | - | TSL:4, |
| USP9X-207 | ENST00000485180.1 | 757 | No protein | Retained intron | - | - | - | TSL:2, |
| USP9X-206 | ENST00000467173.1 | 669 | No protein | Retained intron | - | - | - | TSL:3, |
| USP9X-204 | ENST00000463829.1 | 666 | No protein | Retained intron | - | - | - | TSL:3, |
| USP9X-208 | ENST00000487625.1 | 581 | No protein | Retained intron | - | - | - | TSL:2, |
| USP9X-205 | ENST00000465386.1 | 479 | No protein | Retained intron | - | - | - | TSL:2, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | USP9X |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene USP9X had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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