CRISPR-U™ Gene Knockout Cell Line Strategy

CLTCL1 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human CLTCL1 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol CLTCL1
Gene id 8218
Organism Homo sapiens
Official full symbol clathrin heavy chain like 1
Gene type protein-coding
Also known as CHC22, CLH22, CLTCL, CLTD
Summary This gene is a member of the clathrin heavy chain family and encodes a major protein of the polyhedral coat of coated pits and vesicles. Chromosomal aberrations involving this gene are associated with meningioma, DiGeorge syndrome, and velo-cardio-facial syndrome. Multiple transcript variants encoding different isoforms have been found for this gene.
Genomic regions Chromosome 22
Strategy Summary
This gene has 5 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
CLTCL1-202 ENST00000427926.6 5518 1640aa Protein coding CCDS46662 P53675-1 NM_007098.4 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
CLTCL1-211 ENST00000621271.4 5313 1583aa Protein coding CCDS54497 P53675-2 - TSL:1, GENCODE basic,
CLTCL1-212 ENST00000622493.4 1714 369aa Protein coding - A0A087WV74 - TSL:2, GENCODE basic,
CLTCL1-210 ENST00000617926.4 1695 463aa Protein coding - A0A087WXH4 - TSL:2, GENCODE basic,
CLTCL1-203 ENST00000449918.1 582 169aa Protein coding - F5H5N6 - CDS 3' incomplete, TSL:3,
CLTCL1-209 ENST00000617103.4 4859 1260aa Nonsense mediated decay - A0A087WX41 - TSL:1,
CLTCL1-204 ENST00000458188.2 574 43aa Nonsense mediated decay - H0Y529 - CDS 5' incomplete, TSL:3,
CLTCL1-205 ENST00000538828.1 448 67aa Nonsense mediated decay - H0YGJ9 - CDS 5' incomplete, TSL:5,
CLTCL1-208 ENST00000615606.4 4994 No protein Retained intron - - - TSL:1,
CLTCL1-201 ENST00000412649.5 4635 No protein Retained intron - - - TSL:2,
CLTCL1-207 ENST00000611723.1 838 No protein Retained intron - - - TSL:2,
CLTCL1-206 ENST00000540896.1 477 No protein Retained intron - - - TSL:4,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene CLTCL1
This KO Strategy loading
Red Cotton™ Notes Gene CLTCL1 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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