CRISPR-U™ Gene Knockout Cell Line
Strategy
CLTCL1 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human CLTCL1 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | CLTCL1 |
| Gene id | 8218 |
| Organism | Homo sapiens |
| Official full symbol | clathrin heavy chain like 1 |
| Gene type | protein-coding |
| Also known as | CHC22, CLH22, CLTCL, CLTD |
| Summary | This gene is a member of the clathrin heavy chain family and encodes a major protein of the polyhedral coat of coated pits and vesicles. Chromosomal aberrations involving this gene are associated with meningioma, DiGeorge syndrome, and velo-cardio-facial syndrome. Multiple transcript variants encoding different isoforms have been found for this gene. |
| Genomic regions | Chromosome 22 |
Strategy Summary
This gene has 5 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| CLTCL1-202 | ENST00000427926.6 | 5518 | 1640aa | Protein coding | CCDS46662 | P53675-1 | NM_007098.4 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| CLTCL1-211 | ENST00000621271.4 | 5313 | 1583aa | Protein coding | CCDS54497 | P53675-2 | - | TSL:1, GENCODE basic, |
| CLTCL1-212 | ENST00000622493.4 | 1714 | 369aa | Protein coding | - | A0A087WV74 | - | TSL:2, GENCODE basic, |
| CLTCL1-210 | ENST00000617926.4 | 1695 | 463aa | Protein coding | - | A0A087WXH4 | - | TSL:2, GENCODE basic, |
| CLTCL1-203 | ENST00000449918.1 | 582 | 169aa | Protein coding | - | F5H5N6 | - | CDS 3' incomplete, TSL:3, |
| CLTCL1-209 | ENST00000617103.4 | 4859 | 1260aa | Nonsense mediated decay | - | A0A087WX41 | - | TSL:1, |
| CLTCL1-204 | ENST00000458188.2 | 574 | 43aa | Nonsense mediated decay | - | H0Y529 | - | CDS 5' incomplete, TSL:3, |
| CLTCL1-205 | ENST00000538828.1 | 448 | 67aa | Nonsense mediated decay | - | H0YGJ9 | - | CDS 5' incomplete, TSL:5, |
| CLTCL1-208 | ENST00000615606.4 | 4994 | No protein | Retained intron | - | - | - | TSL:1, |
| CLTCL1-201 | ENST00000412649.5 | 4635 | No protein | Retained intron | - | - | - | TSL:2, |
| CLTCL1-207 | ENST00000611723.1 | 838 | No protein | Retained intron | - | - | - | TSL:2, |
| CLTCL1-206 | ENST00000540896.1 | 477 | No protein | Retained intron | - | - | - | TSL:4, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | CLTCL1 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene CLTCL1 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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