CRISPR-U™ Gene Knockout Cell Line
Strategy
CANX Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human CANX Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | CANX |
| Gene id | 821 |
| Organism | Homo sapiens |
| Official full symbol | calnexin |
| Gene type | protein-coding |
| Also known as | CNX, IP90, P90 |
| Summary | This gene encodes a member of the calnexin family of molecular chaperones. The encoded protein is a calcium-binding, endoplasmic reticulum (ER)-associated protein that interacts transiently with newly synthesized N-linked glycoproteins, facilitating protein folding and assembly. It may also play a central role in the quality control of protein folding by retaining incorrectly folded protein subunits within the ER for degradation. Alternatively spliced transcript variants encoding different isoforms have been described. |
| Genomic regions | Chromosome 5 |
Strategy Summary
This gene has 30 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| CANX-234 | ENST00000681168.1 | 5562 | 592aa | Protein coding | CCDS4447 | - | - | GENCODE basic, APPRIS P2, |
| CANX-219 | ENST00000680013.1 | 5436 | 592aa | Protein coding | CCDS4447 | - | - | GENCODE basic, APPRIS P2, |
| CANX-243 | ENST00000681712.1 | 5427 | 592aa | Protein coding | CCDS4447 | - | - | GENCODE basic, APPRIS P2, |
| CANX-247 | ENST00000681903.1 | 5356 | 592aa | Protein coding | CCDS4447 | - | - | GENCODE basic, APPRIS P2, |
| CANX-210 | ENST00000513246.6 | 5162 | 592aa | Protein coding | CCDS4447 | D6RAQ8 | - | TSL:5, GENCODE basic, APPRIS P2, |
| CANX-220 | ENST00000680042.1 | 5143 | 592aa | Protein coding | CCDS4447 | - | - | GENCODE basic, APPRIS P2, |
| CANX-221 | ENST00000680092.1 | 5105 | 592aa | Protein coding | CCDS4447 | - | - | GENCODE basic, APPRIS P2, |
| CANX-232 | ENST00000681072.1 | 5103 | 592aa | Protein coding | CCDS4447 | - | - | GENCODE basic, APPRIS P2, |
| CANX-244 | ENST00000681733.1 | 4992 | 592aa | Protein coding | CCDS4447 | - | - | GENCODE basic, APPRIS P2, |
| CANX-201 | ENST00000247461.9 | 4915 | 592aa | Protein coding | CCDS4447 | P27824-1 | NM_001746.4 | TSL:1, GENCODE basic, APPRIS P2, MANE Select v0.92, |
| CANX-242 | ENST00000681674.1 | 4910 | 592aa | Protein coding | CCDS4447 | - | - | GENCODE basic, APPRIS P2, |
| CANX-225 | ENST00000680618.1 | 4868 | 592aa | Protein coding | CCDS4447 | - | - | GENCODE basic, APPRIS P2, |
| CANX-239 | ENST00000681476.1 | 4822 | 592aa | Protein coding | CCDS4447 | - | - | GENCODE basic, APPRIS P2, |
| CANX-203 | ENST00000502296.6 | 4483 | 592aa | Protein coding | CCDS4447 | D6RAU8 | - | TSL:4, GENCODE basic, APPRIS P2, |
| CANX-202 | ENST00000452673.6 | 4207 | 592aa | Protein coding | CCDS4447 | P27824-1 | - | TSL:1, GENCODE basic, APPRIS P2, |
| CANX-209 | ENST00000509563.2 | 2515 | 592aa | Protein coding | CCDS4447 | D6RFW4 | - | TSL:4, GENCODE basic, APPRIS P2, |
| CANX-207 | ENST00000504734.5 | 2232 | 592aa | Protein coding | CCDS4447 | P27824-1 | - | TSL:2, GENCODE basic, APPRIS P2, |
| CANX-233 | ENST00000681076.1 | 5260 | 591aa | Protein coding | - | - | - | GENCODE basic, APPRIS ALT1, |
| CANX-224 | ENST00000680614.1 | 5103 | 600aa | Protein coding | - | - | - | GENCODE basic, |
| CANX-230 | ENST00000680984.1 | 5030 | 626aa | Protein coding | - | - | - | GENCODE basic, |
| CANX-246 | ENST00000681894.1 | 4829 | 590aa | Protein coding | - | - | - | GENCODE basic, |
| CANX-222 | ENST00000680406.1 | 4787 | 591aa | Protein coding | - | - | - | GENCODE basic, APPRIS ALT1, |
| CANX-229 | ENST00000680894.1 | 4768 | 591aa | Protein coding | - | - | - | GENCODE basic, APPRIS ALT1, |
| CANX-216 | ENST00000679642.1 | 4722 | 520aa | Protein coding | - | - | - | GENCODE basic, |
| CANX-218 | ENST00000680006.1 | 4663 | 484aa | Protein coding | - | - | - | GENCODE basic, |
| CANX-227 | ENST00000680827.1 | 4622 | 484aa | Protein coding | - | - | - | GENCODE basic, |
| CANX-235 | ENST00000681265.1 | 4308 | 425aa | Protein coding | - | - | - | GENCODE basic, |
| CANX-226 | ENST00000680812.1 | 4157 | 550aa | Protein coding | - | - | - | GENCODE basic, |
| CANX-208 | ENST00000506654.6 | 3934 | 484aa | Protein coding | - | D6RFL1 | - | TSL:3, GENCODE basic, |
| CANX-205 | ENST00000502673.6 | 2171 | 528aa | Protein coding | - | D6RGY2 | - | TSL:5, GENCODE basic, |
| CANX-215 | ENST00000679527.1 | 5417 | 96aa | Nonsense mediated decay | - | - | - | - |
| CANX-245 | ENST00000681762.1 | 4992 | 244aa | Nonsense mediated decay | - | - | - | - |
| CANX-240 | ENST00000681504.1 | 4934 | 471aa | Nonsense mediated decay | - | - | - | - |
| CANX-241 | ENST00000681576.1 | 4882 | 210aa | Nonsense mediated decay | - | - | - | - |
| CANX-231 | ENST00000680985.1 | 4854 | 198aa | Nonsense mediated decay | - | - | - | - |
| CANX-213 | ENST00000679471.1 | 4853 | 58aa | Nonsense mediated decay | - | - | - | - |
| CANX-228 | ENST00000680837.1 | 4799 | 198aa | Nonsense mediated decay | - | - | - | - |
| CANX-223 | ENST00000680504.1 | 4738 | 550aa | Nonsense mediated decay | - | - | - | - |
| CANX-238 | ENST00000681431.1 | 4735 | 69aa | Nonsense mediated decay | - | - | - | - |
| CANX-237 | ENST00000681398.1 | 4687 | 61aa | Nonsense mediated decay | - | D6RG29 | - | - |
| CANX-211 | ENST00000514032.5 | 1876 | 96aa | Nonsense mediated decay | - | D6RD16 | - | TSL:2, |
| CANX-206 | ENST00000503303.1 | 345 | No protein | Processed transcript | - | - | - | TSL:5, |
| CANX-214 | ENST00000679523.1 | 7183 | No protein | Retained intron | - | - | - | - |
| CANX-217 | ENST00000679719.1 | 5562 | No protein | Retained intron | - | - | - | - |
| CANX-212 | ENST00000679395.1 | 5525 | No protein | Retained intron | - | - | - | - |
| CANX-236 | ENST00000681342.1 | 4635 | No protein | Retained intron | - | - | - | - |
| CANX-204 | ENST00000502498.6 | 855 | No protein | Retained intron | - | - | - | TSL:4, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | CANX |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene CANX had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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