CRISPR-U™ Gene Knockout Cell Line Strategy

TRIM56 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human TRIM56 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol TRIM56
Gene id 81844
Organism Homo sapiens
Official full symbol tripartite motif containing 56
Gene type protein-coding
Also known as RNF109
Genomic regions Chromosome 7
Strategy Summary
This gene has 3 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
TRIM56-201 ENST00000306085.11 10910 755aa Protein coding CCDS43625 Q9BRZ2-1 NM_030961.3 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
TRIM56-202 ENST00000412507.1 1129 305aa Protein coding - C9JI91 - CDS 3' incomplete, TSL:1,
TRIM56-203 ENST00000467847.1 581 65aa Protein coding - A0A0D9SF19 - CDS 3' incomplete, TSL:2,
TRIM56-204 ENST00000487252.1 735 No protein Processed transcript - - - TSL:3,
Ubigene Red Cotton Transcript
Strategy
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene TRIM56
This KO Strategy loading
Red Cotton™ Notes Gene TRIM56 had been KO in a549 cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:

$49

Single gRNA plasmid off-shelf

$599

Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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