CRISPR-U™ Gene Knockout Cell Line Strategy

CAMK2G Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human CAMK2G Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol CAMK2G
Gene id 818
Organism Homo sapiens
Official full symbol calcium/calmodulin dependent protein kinase II gamma
Gene type protein-coding
Also known as CAMK, CAMK-II, CAMKG, MRD59
Summary The product of this gene is one of the four subunits of an enzyme which belongs to the serine/threonine protein kinase family, and to the Ca(2+)/calmodulin-dependent protein kinase subfamily. Calcium signaling is crucial for several aspects of plasticity at glutamatergic synapses. In mammalian cells the enzyme is composed of four different chains: alpha, beta, gamma, and delta. The product of this gene is a gamma chain. Many alternatively spliced transcripts encoding different isoforms have been described but the full-length nature of all the variants has not been determined.
Genomic regions Chromosome 10
Strategy Summary
This gene has 10 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
CAMK2G-203 ENST00000322680.7 3818 556aa Protein coding CCDS7338 A0A2Q3DQE3 - TSL:5, GENCODE basic,
CAMK2G-202 ENST00000322635.7 3720 527aa Protein coding CCDS7337 Q13555-5 - TSL:1, GENCODE basic, APPRIS P3,
CAMK2G-204 ENST00000351293.7 3563 495aa Protein coding CCDS7336 Q13555-10 - TSL:5, GENCODE basic, APPRIS ALT1,
CAMK2G-215 ENST00000680035.1 1750 539aa Protein coding CCDS73153 - - GENCODE basic, APPRIS ALT1,
CAMK2G-207 ENST00000423381.6 3878 588aa Protein coding - Q13555-1 NM_001367534.1 TSL:5, GENCODE basic, APPRIS ALT1, MANE Select v0.92,
CAMK2G-209 ENST00000441192.2 2423 588aa Protein coding - H0Y6G2 - TSL:5, GENCODE basic, APPRIS ALT1,
CAMK2G-206 ENST00000394762.7 1893 288aa Protein coding - Q13555-11 - TSL:1, GENCODE basic,
CAMK2G-205 ENST00000372765.5 1822 516aa Protein coding - Q5SWX3 - TSL:5, GENCODE basic, APPRIS ALT1,
CAMK2G-201 ENST00000305762.11 1820 529aa Protein coding - Q13555-4 - TSL:5, GENCODE basic, APPRIS ALT1,
CAMK2G-208 ENST00000433289.5 1618 453aa Protein coding - Q8WU40 - CDS 5' incomplete, TSL:1,
CAMK2G-210 ENST00000472912.5 2629 No protein Processed transcript - - - TSL:2,
CAMK2G-212 ENST00000477205.2 1555 No protein Processed transcript - - - TSL:2,
CAMK2G-214 ENST00000492020.5 773 No protein Processed transcript - - - TSL:5,
CAMK2G-213 ENST00000477652.1 747 No protein Processed transcript - - - TSL:5,
CAMK2G-211 ENST00000474131.5 512 No protein Processed transcript - - - TSL:1,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene CAMK2G
This KO Strategy loading
Red Cotton™ Notes Gene CAMK2G had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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