CRISPR-U™ Gene Knockout Cell Line
Strategy
CAMK2G Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human CAMK2G Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | CAMK2G |
| Gene id | 818 |
| Organism | Homo sapiens |
| Official full symbol | calcium/calmodulin dependent protein kinase II gamma |
| Gene type | protein-coding |
| Also known as | CAMK, CAMK-II, CAMKG, MRD59 |
| Summary | The product of this gene is one of the four subunits of an enzyme which belongs to the serine/threonine protein kinase family, and to the Ca(2+)/calmodulin-dependent protein kinase subfamily. Calcium signaling is crucial for several aspects of plasticity at glutamatergic synapses. In mammalian cells the enzyme is composed of four different chains: alpha, beta, gamma, and delta. The product of this gene is a gamma chain. Many alternatively spliced transcripts encoding different isoforms have been described but the full-length nature of all the variants has not been determined. |
| Genomic regions | Chromosome 10 |
Strategy Summary
This gene has 10 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| CAMK2G-203 | ENST00000322680.7 | 3818 | 556aa | Protein coding | CCDS7338 | A0A2Q3DQE3 | - | TSL:5, GENCODE basic, |
| CAMK2G-202 | ENST00000322635.7 | 3720 | 527aa | Protein coding | CCDS7337 | Q13555-5 | - | TSL:1, GENCODE basic, APPRIS P3, |
| CAMK2G-204 | ENST00000351293.7 | 3563 | 495aa | Protein coding | CCDS7336 | Q13555-10 | - | TSL:5, GENCODE basic, APPRIS ALT1, |
| CAMK2G-215 | ENST00000680035.1 | 1750 | 539aa | Protein coding | CCDS73153 | - | - | GENCODE basic, APPRIS ALT1, |
| CAMK2G-207 | ENST00000423381.6 | 3878 | 588aa | Protein coding | - | Q13555-1 | NM_001367534.1 | TSL:5, GENCODE basic, APPRIS ALT1, MANE Select v0.92, |
| CAMK2G-209 | ENST00000441192.2 | 2423 | 588aa | Protein coding | - | H0Y6G2 | - | TSL:5, GENCODE basic, APPRIS ALT1, |
| CAMK2G-206 | ENST00000394762.7 | 1893 | 288aa | Protein coding | - | Q13555-11 | - | TSL:1, GENCODE basic, |
| CAMK2G-205 | ENST00000372765.5 | 1822 | 516aa | Protein coding | - | Q5SWX3 | - | TSL:5, GENCODE basic, APPRIS ALT1, |
| CAMK2G-201 | ENST00000305762.11 | 1820 | 529aa | Protein coding | - | Q13555-4 | - | TSL:5, GENCODE basic, APPRIS ALT1, |
| CAMK2G-208 | ENST00000433289.5 | 1618 | 453aa | Protein coding | - | Q8WU40 | - | CDS 5' incomplete, TSL:1, |
| CAMK2G-210 | ENST00000472912.5 | 2629 | No protein | Processed transcript | - | - | - | TSL:2, |
| CAMK2G-212 | ENST00000477205.2 | 1555 | No protein | Processed transcript | - | - | - | TSL:2, |
| CAMK2G-214 | ENST00000492020.5 | 773 | No protein | Processed transcript | - | - | - | TSL:5, |
| CAMK2G-213 | ENST00000477652.1 | 747 | No protein | Processed transcript | - | - | - | TSL:5, |
| CAMK2G-211 | ENST00000474131.5 | 512 | No protein | Processed transcript | - | - | - | TSL:1, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | CAMK2G |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene CAMK2G had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
Comment: