CRISPR-U™ Gene Knockout Cell Line
Strategy
HMGA2 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human HMGA2 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | HMGA2 |
| Gene id | 8091 |
| Organism | Homo sapiens |
| Official full symbol | high mobility group AT-hook 2 |
| Gene type | protein-coding |
| Also known as | BABL, HMGI-C, HMGIC, LIPO, SRS5, STQTL9 |
| Summary | This gene encodes a protein that belongs to the non-histone chromosomal high mobility group (HMG) protein family. HMG proteins function as architectural factors and are essential components of the enhancesome. This protein contains structural DNA-binding domains and may act as a transcriptional regulating factor. Identification of the deletion, amplification, and rearrangement of this gene that are associated with myxoid liposarcoma suggests a role in adipogenesis and mesenchymal differentiation. A gene knock out study of the mouse counterpart demonstrated that this gene is involved in diet-induced obesity. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. |
| Genomic regions | Chromosome 12 |
Strategy Summary
This gene has 7 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| HMGA2-204 | ENST00000403681.7 | 4117 | 109aa | Protein coding | CCDS44936 | P52926-1 | NM_003483.6 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| HMGA2-203 | ENST00000393578.7 | 1993 | 90aa | Protein coding | CCDS81709 | P52926-6 | - | TSL:1, GENCODE basic, |
| HMGA2-206 | ENST00000536545.5 | 1788 | 147aa | Protein coding | CCDS73491 | F5H6H0 | - | TSL:1, GENCODE basic, |
| HMGA2-201 | ENST00000354636.7 | 1529 | 106aa | Protein coding | CCDS31854 | P52926-2 | - | TSL:1, GENCODE basic, |
| HMGA2-202 | ENST00000393577.7 | 611 | 118aa | Protein coding | CCDS73492 | F5H2A4 | - | TSL:3, GENCODE basic, |
| HMGA2-210 | ENST00000541363.5 | 8094 | 99aa | Protein coding | - | F5H2U8 | - | TSL:1, GENCODE basic, |
| HMGA2-205 | ENST00000425208.6 | 1444 | 92aa | Protein coding | - | P52926-4 | - | TSL:1, GENCODE basic, |
| HMGA2-208 | ENST00000537429.5 | 1527 | 86aa | Nonsense mediated decay | - | P52926-3 | - | TSL:1, |
| HMGA2-207 | ENST00000537275.5 | 1508 | 103aa | Nonsense mediated decay | - | P52926-5 | - | TSL:1, |
| HMGA2-209 | ENST00000539662.1 | 485 | 55aa | Nonsense mediated decay | - | H0YFY4 | - | CDS 5' incomplete, TSL:3, |
| HMGA2-211 | ENST00000545998.1 | 1584 | No protein | Retained intron | - | - | - | TSL:1, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | HMGA2 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene HMGA2 had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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