CRISPR-U™ Gene Knockout Cell Line Strategy

HMGA2 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human HMGA2 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol HMGA2
Gene id 8091
Organism Homo sapiens
Official full symbol high mobility group AT-hook 2
Gene type protein-coding
Summary This gene encodes a protein that belongs to the non-histone chromosomal high mobility group (HMG) protein family. HMG proteins function as architectural factors and are essential components of the enhancesome. This protein contains structural DNA-binding domains and may act as a transcriptional regulating factor. Identification of the deletion, amplification, and rearrangement of this gene that are associated with myxoid liposarcoma suggests a role in adipogenesis and mesenchymal differentiation. A gene knock out study of the mouse counterpart demonstrated that this gene is involved in diet-induced obesity. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
Genomic regions Chromosome 12
Strategy Summary
This gene has 7 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
HMGA2-204 ENST00000403681.7 4117 109aa Protein coding CCDS44936 P52926-1 NM_003483.6 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
HMGA2-203 ENST00000393578.7 1993 90aa Protein coding CCDS81709 P52926-6 - TSL:1, GENCODE basic,
HMGA2-206 ENST00000536545.5 1788 147aa Protein coding CCDS73491 F5H6H0 - TSL:1, GENCODE basic,
HMGA2-201 ENST00000354636.7 1529 106aa Protein coding CCDS31854 P52926-2 - TSL:1, GENCODE basic,
HMGA2-202 ENST00000393577.7 611 118aa Protein coding CCDS73492 F5H2A4 - TSL:3, GENCODE basic,
HMGA2-210 ENST00000541363.5 8094 99aa Protein coding - F5H2U8 - TSL:1, GENCODE basic,
HMGA2-205 ENST00000425208.6 1444 92aa Protein coding - P52926-4 - TSL:1, GENCODE basic,
HMGA2-208 ENST00000537429.5 1527 86aa Nonsense mediated decay - P52926-3 - TSL:1,
HMGA2-207 ENST00000537275.5 1508 103aa Nonsense mediated decay - P52926-5 - TSL:1,
HMGA2-209 ENST00000539662.1 485 55aa Nonsense mediated decay - H0YFY4 - CDS 5' incomplete, TSL:3,
HMGA2-211 ENST00000545998.1 1584 No protein Retained intron - - - TSL:1,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene HMGA2
This KO Strategy loading
Red Cotton™ Notes Gene HMGA2 had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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