CRISPR-U™ Gene Knockout Cell Line
Strategy
ILKAP Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human ILKAP Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | ILKAP |
| Gene id | 80895 |
| Organism | Homo sapiens |
| Official full symbol | ILK associated serine/threonine phosphatase |
| Gene type | protein-coding |
| Also known as | ILKAP2, ILKAP3, PP2C-DELTA, PP2CD, PPM1O |
| Summary | The protein encoded by this gene is a protein serine/threonine phosphatase of the PP2C family. This protein can interact with integrin-linked kinase (ILK/ILK1), a regulator of integrin mediated signaling, and regulate the kinase activity of ILK. Through the interaction with ILK, this protein may selectively affect the signaling process of ILK-mediated glycogen synthase kinase 3 beta (GSK3beta), and thus participate in Wnt signaling pathway. |
| Genomic regions | Chromosome 2 |
Strategy Summary
This gene has 5 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| ILKAP-201 | ENST00000254654.8 | 1455 | 392aa | Protein coding | CCDS2526 | Q9H0C8 | NM_030768.3 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| ILKAP-211 | ENST00000612675.4 | 1335 | 162aa | Protein coding | - | F8SNU6 | - | TSL:1, GENCODE basic, |
| ILKAP-212 | ENST00000622223.4 | 1088 | 274aa | Protein coding | - | F8SNU7 | - | TSL:1, GENCODE basic, |
| ILKAP-202 | ENST00000450411.1 | 1024 | 203aa | Protein coding | - | H7C2I8 | - | CDS 5' incomplete, TSL:2, |
| ILKAP-203 | ENST00000457149.1 | 793 | 212aa | Protein coding | - | E9PC05 | - | CDS 3' incomplete, TSL:5, |
| ILKAP-209 | ENST00000490837.1 | 557 | No protein | Processed transcript | - | - | - | TSL:4, |
| ILKAP-206 | ENST00000466468.5 | 2651 | No protein | Retained intron | - | - | - | TSL:2, |
| ILKAP-204 | ENST00000463129.5 | 1861 | No protein | Retained intron | - | - | - | TSL:2, |
| ILKAP-205 | ENST00000465131.5 | 750 | No protein | Retained intron | - | - | - | TSL:2, |
| ILKAP-208 | ENST00000479400.1 | 690 | No protein | Retained intron | - | - | - | TSL:3, |
| ILKAP-207 | ENST00000467133.5 | 618 | No protein | Retained intron | - | - | - | TSL:4, |
| ILKAP-210 | ENST00000498727.5 | 587 | No protein | Retained intron | - | - | - | TSL:2, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | ILKAP |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene ILKAP had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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