CRISPR-U™ Gene Knockout Cell Line Strategy

ILKAP Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human ILKAP Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol ILKAP
Gene id 80895
Organism Homo sapiens
Official full symbol ILK associated serine/threonine phosphatase
Gene type protein-coding
Also known as ILKAP2, ILKAP3, PP2C-DELTA, PP2CD, PPM1O
Summary The protein encoded by this gene is a protein serine/threonine phosphatase of the PP2C family. This protein can interact with integrin-linked kinase (ILK/ILK1), a regulator of integrin mediated signaling, and regulate the kinase activity of ILK. Through the interaction with ILK, this protein may selectively affect the signaling process of ILK-mediated glycogen synthase kinase 3 beta (GSK3beta), and thus participate in Wnt signaling pathway.
Genomic regions Chromosome 2
Strategy Summary
This gene has 5 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
ILKAP-201 ENST00000254654.8 1455 392aa Protein coding CCDS2526 Q9H0C8 NM_030768.3 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
ILKAP-211 ENST00000612675.4 1335 162aa Protein coding - F8SNU6 - TSL:1, GENCODE basic,
ILKAP-212 ENST00000622223.4 1088 274aa Protein coding - F8SNU7 - TSL:1, GENCODE basic,
ILKAP-202 ENST00000450411.1 1024 203aa Protein coding - H7C2I8 - CDS 5' incomplete, TSL:2,
ILKAP-203 ENST00000457149.1 793 212aa Protein coding - E9PC05 - CDS 3' incomplete, TSL:5,
ILKAP-209 ENST00000490837.1 557 No protein Processed transcript - - - TSL:4,
ILKAP-206 ENST00000466468.5 2651 No protein Retained intron - - - TSL:2,
ILKAP-204 ENST00000463129.5 1861 No protein Retained intron - - - TSL:2,
ILKAP-205 ENST00000465131.5 750 No protein Retained intron - - - TSL:2,
ILKAP-208 ENST00000479400.1 690 No protein Retained intron - - - TSL:3,
ILKAP-207 ENST00000467133.5 618 No protein Retained intron - - - TSL:4,
ILKAP-210 ENST00000498727.5 587 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene ILKAP
This KO Strategy loading
Red Cotton™ Notes Gene ILKAP had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

Please leave your suggestion ×