CRISPR-U™ Gene Knockout Cell Line
Strategy
SETD7 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human SETD7 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | SETD7 |
| Gene id | 80854 |
| Organism | Homo sapiens |
| Official full symbol | SET domain containing 7, histone lysine methyltransferase |
| Gene type | protein-coding |
| Also known as | KMT7, SET7, SET7/9, SET9 |
| Genomic regions | Chromosome 4 |
Strategy Summary
This gene has 6 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| SETD7-201 | ENST00000274031.8 | 6808 | 366aa | Protein coding | CCDS3748 | Q8WTS6 | NM_030648.4 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| SETD7-204 | ENST00000506866.6 | 1602 | 362aa | Protein coding | CCDS77961 | D6RJA0 | - | TSL:1, GENCODE basic, |
| SETD7-202 | ENST00000404104.7 | 1590 | 135aa | Protein coding | CCDS82956 | B5MCZ8 | - | TSL:2, GENCODE basic, |
| SETD7-207 | ENST00000608958.1 | 5281 | 46aa | Protein coding | - | V9GY19 | - | TSL:5, GENCODE basic, |
| SETD7-203 | ENST00000406354.1 | 1127 | 69aa | Protein coding | - | B5MCS2 | - | TSL:1, GENCODE basic, |
| SETD7-206 | ENST00000608795.5 | 492 | 94aa | Protein coding | - | V9GYG1 | - | CDS 3' incomplete, TSL:2, |
| SETD7-205 | ENST00000515101.1 | 711 | No protein | Retained intron | - | - | - | TSL:3, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | SETD7 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene SETD7 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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