CRISPR-U™ Gene Knockout Cell Line Strategy

SETD7 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human SETD7 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol SETD7
Gene id 80854
Organism Homo sapiens
Official full symbol SET domain containing 7, histone lysine methyltransferase
Gene type protein-coding
Also known as KMT7, SET7, SET7/9, SET9
Genomic regions Chromosome 4
Strategy Summary
This gene has 6 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
SETD7-201 ENST00000274031.8 6808 366aa Protein coding CCDS3748 Q8WTS6 NM_030648.4 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
SETD7-204 ENST00000506866.6 1602 362aa Protein coding CCDS77961 D6RJA0 - TSL:1, GENCODE basic,
SETD7-202 ENST00000404104.7 1590 135aa Protein coding CCDS82956 B5MCZ8 - TSL:2, GENCODE basic,
SETD7-207 ENST00000608958.1 5281 46aa Protein coding - V9GY19 - TSL:5, GENCODE basic,
SETD7-203 ENST00000406354.1 1127 69aa Protein coding - B5MCS2 - TSL:1, GENCODE basic,
SETD7-206 ENST00000608795.5 492 94aa Protein coding - V9GYG1 - CDS 3' incomplete, TSL:2,
SETD7-205 ENST00000515101.1 711 No protein Retained intron - - - TSL:3,
Ubigene Red Cotton Transcript
Strategy
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene SETD7
This KO Strategy loading
Red Cotton™ Notes Gene SETD7 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
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Work flow
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